摘要
目的:建立以高效液相色谱法测定活血通脉片中人参皂苷Rg1含量的方法。方法:色谱柱为SHIP-PACK VP ODS(150mm×4.6mm,5μm),流动相为乙腈-水(28:72),流速为1.0mL.min-1,检测波长为210nm,柱温为25℃,进样量为20μL。结果:人参皂苷Rg1检测浓度在40.0～200.0μg.mL-1范围内与峰面积积分值呈良好的线性关系(r=0.9999);平均加样回收率为99.95%,RSD=6.78%(n=6)。结论:本方法可靠、灵敏、准确、易行,可用于活血通脉片的质量控制。
OBJECTIVE： To establish a HPLC method for the determination of the content of Ginsenoside Rg, in Huoxue tongmai tablets. METHODS： The analytical column was SHIP-PACK VP ODS（ 150mm ×4.6mm, 5μm）with column temperature at 25℃, and the mobile phase was composed of acetonitrile-water（28 ： 72）with flow rate at 1.0 mL·min^-1. The detection wavelength was set at 210nm and the sample size was 20μL. RESULTS： The linear range of Ginsenoside Rg, was 40.0-- 200.0μg·mL^-1（ r = 0.999 9）, and its average recovery was 99.95%, RSD = 6.78% （n = 6） . CONCLUSION： This method is reliable, sensitive, accurate and easy to operate, and it can be used as a quality control of Huoxue tongmai tablets.
出处
《中国药房》
CAS
CSCD
北大核心
2007年第24期1880-1881,共2页
China Pharmacy
