摘要
目的建立全血中雷米芬太尼的高效液相色谱/质谱(HPLC/MS)测定方法,并测定围麻醉期患者体内雷米芬太尼的血药浓度。方法全血样品用1-氯丁烷进行提取,以50%甲醇含乙酸铵(20mmol/L)为流动相,色谱柱为SunfireTMC-18柱(2.1mm×150mm,5μm),流速0.3ml/min。质谱采用电喷雾电离(ESI)方式,正离子采集,以选择离子(SIR)方式进行定量,检测离子为雷米芬太尼377。结果芬太尼在0.5～70.0ng/ml浓度范围内呈良好的线性(r=0.9995)。日内、日间精密度均在15%以内,提取回收率大于82.3%,方法回收率在97.62%～100.58%。结论HPLC/MS方法灵敏、准确,适合雷米芬太尼的体内药物浓度测定。
Objective To establish a HPLC/MS method for the determination of blood remifentanil concentration. Methods The blood samples extracted with 1-chlorobutane were separated over an Sunfire^TM C-18 column （2.1 mm× 150 mm, 5μm） and the mobile phase consisted of a mixture of 50% mathanol containing ammonium acetate in concentration of 20 mmol/L. The flow rate was 0.3 ml/min. ESI＋, and SIR were used for quantification. The selected ions were 377 for remifentanil. Results The calibration linear range of blood remifentanil concentrations was 0.5-70 ng/ml （r= 0. 9995）. The between-day and within-day RSD% as less than 15 %. The extraction recoveries were more than 82.3% and the method recoveries were between 97.62% and 100. 58%. Conclusion HPLC/MS method is rapid, sensitive and reliable for monitoring blood remifentanil concentration.
出处
《江苏医药》
CAS
CSCD
北大核心
2007年第7期718-720,共3页
Jiangsu Medical Journal
