摘要
目的 :建立测定人血中瑞芬太尼的高效液相色谱 紫外检测方法 ,为临床监测血药浓度提供方法学。方法 :采用液 液萃取法 ,用 1 氯丁烷提取全血中的瑞芬太尼 ,选用GI97559(瑞芬太尼乙基类似物 )为内标定量。色谱柱为ZorbaxSB -CN(4.6mm× 2 50mm ,5μm) ,流动相为乙腈 甲醇 磷酸二氢钠缓冲液 (1∶2∶0 .43) ,柱温 30℃ ,流速 1 .5mL·min- 1 ,紫外 2 1 0nm检测 ,按内标法定量。结果 :标准曲线在 3～ 2 0 0 μg·L- 1 范围内 ,浓度与峰面积比有良好的线性关系 (r =0 .9966)。最低检测浓度为 2 μg·L- 1 。提取回收率 77.34 %～ 87.72 %。方法回收率 97.1 3 %～ 1 0 0 .99% ,日内RSD小于 5 .3 % ,日间RSD小于6 .3 %。结论 :本方法快速、准确、灵敏 ,可用于瑞芬太尼的临床药动学研究。
OBJECTIVE An HPLC-UV method was established for determination of remifentanil in whole blood. METHODS The new HPLC procedure involved a liquid-liquid extraction. The remifentanil in blood was extracted with n-butyl chloride, and GI97559-an ethyl analogue of the drug was used as the internal standard. The sample was separated on Zorbax SB-CN( 4.6 mm×250 mm, 5 μm)colum with a mobile phase of mixture of acetonitrile, methanol (1∶2) and phosphate buffer, the temperature was set at 30℃ with flow rate of 1.5 mL·min -1 , detection -UV at 210 nm . RESULTS A linearity was obtained from 3 ng·mL -1 to 200 μg·L -1 of remifetanil in whole blood with a good correlation ( r = 0.9966 ). Detection limit was 2 μg ·L -1 .The recovery of extraction was 77.34% ～ 87.22% . The recovery of methodology was 97.13% ～ 100.99% ,within-day RSD less than 5.3% ; inter-day RSD less than 6.3% .CONCLUSIONS This method is rapid, accurate and sensitive, it is very suitable for the investigation of clinical pharmacokinetics of remifentanil.
出处
《中国医院药学杂志》
CAS
CSCD
北大核心
2004年第2期67-68,共2页
Chinese Journal of Hospital Pharmacy
