摘要
目的研究大鼠脂肪干细胞的生物学特性,为外周神经组织工程应用提供实验基础。方法从F344大鼠的脂肪组织分离得到脂肪干细胞,体外培养并扩增,倒置相差显微镜观察其形态、绘制生长曲线研究增殖能力,流式细胞仪检测表面标志,核型分析研究遗传学性能,最后以5-溴脱氧尿嘧啶核苷(BrdU)进行体外标记。结果脂肪干细胞形态以梭形为主,CD11b、CD45、CD49d、CD80、CD86表达阴性,MHC I、MHCⅡ表达弱阳性,CD29、CD44、CD54的表达阳性。第11代以前的脂肪干细胞有较强的活力和增殖能力,体外培养至第10代,其细胞仍稳定为二倍体核型。BrdU可标记其核。结论脂肪干细胞具有较强的自我更新能力,其形态和表面标志均类似大鼠骨髓间充质干细胞。
Objective To investigate the biological characteristics of rat adipose tissue-derived stromal cells （ADSCs）. Methods ADSCs were isolated from the adipose tissue of adult F344 rat, expanded in culture medium. The morphology was observed by inverted phase contrast microscope, and growth curve was studied. The surface antigens were analysized by flow cytometer, and the hereditary feature was reflected by karyotype analysis. ADSCs were labeled with 5-bromo-2＇-deoxyuridine （ BrdU ）. Results ADSCs were mostly spindle shaped cells. Flow cytometry results showed ADSCs were positive for CD29, CD44, CD55, MHC- Ⅰ and MHC-Ⅱ , and negative for CD11b, CIM5, CD80, CD86. The ceils of prepassage 11 had a strong self-renewal capacity, and the cells of passage 10 still had normal karyotype. BrdU labeled their nuclei. Conclusion ADSCs isolated from the adipose tissue had strong self-renewal capacity. The morphology and surface antigens of ADSCs were similar to rat bone marrow stromal cells.
出处
《中华显微外科杂志》
CSCD
北大核心
2007年第3期189-192,I0002,共5页
Chinese Journal of Microsurgery
基金
广州市科技攻关重大项目(2002ZI-E0035)
广东省科技攻关重大项目(2003A3020102和2004A30201001)
广东省自然科学基金(06021291)
关键词
脂肪干细胞
细胞培养
大鼠
Adipose tissue-derived stromal ceils
Cell culture
Rat
