摘要
目的探讨脂肪干细胞（ADSCs）应用于组织工程化外周神经修复大鼠坐骨神经缺损的效果。方法48只体重200～220g的雌性F344大鼠随机分成6组，每组8只，分别用下面6种不同的实验组修复15mm长坐骨神经缺损。A组：种植ADSCs的去细胞神经；B组：种植诱导ADSCs的去细胞神经；C组：种植许旺细胞（SOs）的去细胞神经；D组：去细胞神经；E组：自体神经移植；F组：空白对照。通过神经电生理检测、荧光金逆行示踪、组织学检测和坐骨神经功能指数测定评价各组修复神经缺损的效果。结果术后12周，F组未见桥接物，A组和B组的神经电生理等各项指标均分别优于D组（P〈0．05或P〈0．01），与C组和E组间差异无统计学意义（P〉0．05）。结论初步结果显示ADSCs及诱导后ADSCs作为种子细胞，与去细胞神经构建的组织工程化外周神经移植体，能够修复外周神经缺损。
Objective To evaluate the effectiveness of using adipose-derived stromal cells （ADSCs） into a tissue-engineered peripheral nerve on bridging sciatic nerve gaps. Methods Forty-eight F344 female rats weighing 200 ～220 g were randomly divided into 6 groups of nerve grafting to repair 15 mm long sciatic nerve lesions, with 8 rats in each group. Group A： ADSCs-laden acellular nerves; group B： differentiated ADSCs-laden acellular nerves; group C： Schwann cells-laden acellular nerves; group D： acellular nerves without cells; group E： autografts; group F： nonimplanted grafts. The effects were evaluated in terms of electrophysiology, Fluorogold retrograde tracing, histology and tracking studies. Results At 12 weeks after surgery, there was no graft bridging nerve gap in nonimplanted grafts. All the examinations of group A and B were better than group D, respectively （P 〈 0.05 or P 〈 0.01）. But there were no statistically significant differences among group A, B, C, and D （P 〉 0.05）. Conclusion ADSCs and differentiated ADSCs could promote nerve regeneration when used as seed cells to build tissue-engineered peripheral nerves with acellular nerve scaffolds.
出处
《中华显微外科杂志》
CSCD
北大核心
2008年第5期350-353,I0007,共5页
Chinese Journal of Microsurgery
基金
广州市科技攻关重大项且（200221-E0035）
国家高科技研究发展（863）计划资助项目（2006AA02A130）
广东省自然科学基金（06021291）
关键词
脂肪干细胞
许旺细胞
组织工程
神经移植
显微外科
Adipose derived stromal cells
Schwann cells
Tissue engineering
Nerve graft
Microsurgery
