摘要
目的 :观察溴脱氧尿嘧啶核苷 (BrdU)标记在肿瘤增殖研究中的价值。方法 :用 80 μg ml的BrdU或碘脱氧尿嘧啶核苷 (IdU)体外掺入Tca8113细胞或舌癌组织 ,标本连续切片与增殖细胞核抗原 (PCNA)表达比较 ;相同孵育时间的细胞与流式细胞术 (FCM)定量S期细胞数比较。结果 :BrdU标记细胞核大浑圆 ,呈散在或姊妹细胞分布 ,子代细胞染色稍淡。BrdU或IdU掺入和PCNA表达之间无显著性差异 (P >0 0 5 )。FCM测定的S期细胞数显著多于BrdU掺入的细胞数 (P <0 0 0 1)。结论 :BrdU标记可用于细胞增殖研究 。
Objective:The aim of this study is to evaluate of Bromodeoxyuridine (BrdU) label in the proliferation of the tongue cancer. Methods:The authors used 80 μg/ml BrdU or iododeoxyuridine (IdU) to label three cases of fresh tissue of tongue cancer and four bottles of Tca8113 cells in logarithmic growth period in vitro. They were fixed and embedded in the routine procedure, and then in serial sections. These specimens were labeled with BrdU polyclonal antibody (1∶200), and PCNA monoclonal antibody (1∶100), stained using immunohistochemical ABC method. The authors observed the shapes and sizes of those positive cells, counted their label indexes (LI) of BrdU_ or IdU_positive cells and PCNA_ positive cells respectively. Four bottles of Tca8113 cells were cultured for 1～4 days. One bottle of the cells was every day used in the experimental study about comparing the cells labeled BrdU with those of the S phase of FCM in same cultural time.Results:The positive cells generally owned a larger round nucleus, sometimes in company with sister cells. There was no difference of positive cells in these two kinds of labeling methods between the incorporation of BrdU or IdU and the expression of proliferating cell nuclear antigen (P>0.05). The cells of S phase with FCM method were much more than that method with BrdU incorporation (P<0.001).Conclusion: The method of BrdU labeling could be used in tumor cellular proliferation studies.\;
出处
《华西口腔医学杂志》
CAS
CSCD
北大核心
2001年第5期278-280,共3页
West China Journal of Stomatology
