摘要
目的利用人乳腺癌T47D细胞观察异补骨脂素和蜕皮甾酮对雌激素受体阳性细胞增殖的作用,并检测其对T47D细胞雌激素受体亚型ERα、ERβ表达的影响。方法采用MTT细胞增殖实验观察中药补骨脂、川牛膝的两种活性成分:异补骨脂素和蜕皮甾酮对ERα、ERβ阳性细胞T47D增殖的影响;通过流式细胞术检测细胞增殖周期的变化;用半定量RT-PCR法检测上述成分对靶细胞ERα、ERβmRNA表达情况的影响。结果10-6mol/L和10-7mol/L异补骨脂素和蜕皮甾酮能够显著促进培养的T47D细胞增殖,提高细胞分裂增殖指数,表现出雌激素样作用;RT-PCR结果显示:10-6mol/L和10-7mol/L异补骨脂素和蜕皮甾酮均可使ERαmRNA表达显著增加,异补骨脂素同时也可提高ERβmRNA表达。结论异补骨脂素和蜕皮甾酮具有植物雌激素作用,并可影响靶细胞ER亚型的mRNA表达水平。
Objective To observe the effects of isopsoralen and ecdysterone on the proliferation of T47D cells and detect their influence on the expressions of T47D subtypes -estrogen receptor α （ERα） and estrogen receptor β （ERβ） cells in human breast cancer. Methods The influence of isopsoralen and eedysterone （the active principles of Fructus Psoraleae, Radix Cyathulae） on the proliferation of ERα and ERβ cells and on the expression levels of ERα and ERβ mRNA by mRNA assay and RT-PCR respectively. The changes of cell proliferation cycle were detected by flow cytometry. Results Isepsoralen and ecdysterone （in dose of 10^-6 mol/L and 10^-7 mol/L respectively） promoted obviously the proliferation of cultured T47D cells, increased the proliferation indexes with an estrogenic effect. The result of RT-PCR showed that isopsoralen （10^-6mol/ L） and ecdysterone （10^-7 mol/L） improved the expression of ERα mRNA and isopsoralen improved the expression of ERβ mRNA at the same time. Conclusion Isopsoralen and ecdysterone have the phytoestrogenic effects and can influence the mRNA expression level of ER subtype.
出处
《北京中医药大学学报》
CAS
CSCD
北大核心
2007年第4期242-245,共4页
Journal of Beijing University of Traditional Chinese Medicine
基金
国家自然科学基金资助项目(No.3051040320230540420032)
教育部长江学者和创新团队发展计划资助项目(No.IRT0413)
教育部博士点科研基金资助项目(No.2005002601220060026019)
