摘要
目的:补骨脂汤改善血管性痴呆大鼠学习记忆的作用。方法:通过双侧颈动脉结扎制作血管性痴呆模型,用RT-PCR及免疫组化方法测定海马内雌激素受体β(ER-β)、NMDAR2B(NR2B)受体基因的表达产物。结果:补骨脂汤组ER-β、NR2B基因表达的mRNA量明显高于模型组及银杏片组(P<0.05);补骨脂汤组大鼠海马CA3区的阳性细胞数均高于模型组、银杏片组(P<0.05)。结论:补骨脂汤能改善血管性痴呆大鼠的学习记忆,可能的作用途径是提高ER-β受体、NR2B受体的基因表达水平,从而提高学习记忆。
Objective：To investigate the mechanism of Buguozhi Decoction in improving learning-memory of vascular dementia model rats.Methods：The rat models with VaD were established by bilateral occlusion of both common carotid arteries（2 一V0）.The gene expressions of ER-β,NR2B were evaluated by RT-PCR and immunohistochemistry.Results：Buguozhi Decoction can significantly improve mRNA and protien pruducts of ER-β,NR2B gene in hippocampus of the vascular dementia model rats,compared with the model group（P0.05）.Conclusion：The mechanism of Buguozhi Decoction in improving the learningmemory of the vascular dementia model rats maybe to increase the expression of ER-βand NR2B gene.
出处
《中华中医药杂志》
CAS
CSCD
北大核心
2010年第9期1496-1498,共3页
China Journal of Traditional Chinese Medicine and Pharmacy
基金
江西省自然科学基金(No.0610159)
江西省教育厅项目(No.GJJ09281)
江西省卫生厅项目(No.2008Z0028)~~
