摘要
目的：建立补骨脂药材中化学成分的高效液相色谱-电喷雾质谱分析方法。方法：采用DiamonsilTMC18（4.6 mm×250 mm,5μm）色谱柱;流动相0.05%甲酸水溶液-乙腈,梯度洗脱;流速1 mL.min-1;柱温30℃;DAD扫描范围190-400nm;检测波长246 nm。Finnigan电喷雾离子阱多级质谱仪;正离子检测模式;ESI喷雾电压4 500 V;鞘气（N2）流速60个单位;辅助气（N2）流速20个单位;毛细管温度350℃;毛细管电压19 V,扫描范围m/z90-800。结果：补骨脂中化学成分获得了较好的分离和检测,共鉴定出2个香豆素苷,3个香豆素,8个黄酮和1个单萜酚类成分。结论：该方法灵敏度高、分离度好,适用于补骨脂药材中化学成分的快速定性鉴定。
Objective： To identify the compounds in fruits of Psoralea corylifolia by high performance liquid chromatographymass spectrometry. Method： The chromatographic separation was carried out at 30℃ on a DiamonsilTM C18 column （4. 6 mm × 250 mm, 5 μm） eluted in the gradient program. The mobile phase consisted of acetonitrile （A） and water containing 0. 05% formic acid （B）. The detection wavelength was at 246 nm and the on-line UV spectra was recorded in the range of 190-400 nm. The mass spectra were obtained by Finnigan LCQ Deca XPplus ion trap mass spectrometer in the positive ion mode with ion spray voltage 4. 5 kV, sheath gas （ N2 ）, 60 arbitrary units, auxiliary gas （ N2 ）, 20 arbitrary units ; capillary temperature, 350 ℃, capillary voltage 19 V, tube lens offset voltage 25 V, mass range recorded, m/z 90-800. Result： Two coumarin glycosides, three coumarins, eight flavonoids and one meroterpene were identified by HPLC-MS method. Conclusion： The method is simple and rapid for the identification and quality control for the fruits of P. corylifolia.
出处
《中国中药杂志》
CAS
CSCD
北大核心
2009年第22期2898-2902,共5页
China Journal of Chinese Materia Medica
基金
国家自然科学基金项目(30701093)
高等学校博士学科点专项科研基金项目(20070063003)
