摘要
目的制备三七总皂苷复方脂质体凝胶剂,并进行体外透皮吸收研究。方法以薄膜分散法制备脂质体,通过正交实验设计优化三七总皂苷复方脂质体的处方,再以卡波姆为基质制成凝胶剂。通过智能透皮试验仪,进行体外透皮实验,研究三七总皂苷中人参皂苷Rg_1及β-蜕皮激素的体外透皮吸收情况,并采用HPLC法检测透过及滞留在皮肤内的Rg_1和β-蜕皮激素量。结果卵磷脂与胆固醇用量比为9:1,Rg_1质量浓度为40 g·L^(-1)β-蜕皮激素质量浓度为3g·L^(-1),脂药比为5:1时的处方为最佳脂质体处方,卡波姆-934重量比为1%时,药物的皮肤滞留量最大。结论将三七总皂苷制备成复方脂质体凝胶剂,能够增加药物的皮肤滞留量,提高药物在皮肤局部的生物利用度。
AIM To prepare saponins of panax notoginseng（PNS）/β-ecdysone（EDS）compound liposomal gel and study the drug percutaneous absorption in vitro. METHODS The PNS/EDS compound liposomes were prepared by the film-dispersion method. The compound liposome formulation was optimized by the orthogonal experimental design and carbomer was added as a supportive base for the preparation of liposomal gel. Drug permeation tests were performed through excised rat skin in diffusion cells. RESULTS The final liposomal formulation was as follows ： lecithin： cholesterin = 9 ： 1, Rgl content was 40 g· L^- 1, EDS content was 3 g· L^- 1, lipid ： drug = 5： 1, and the highest accumulative drug concentration in the skin was obtained when the content of carbopol was 1%. CONCLUSION The liposomal gel has the ability to increase the drug concentration in the excised rat skin, which can improve the bioavailability of the local skin.
出处
《中国临床药学杂志》
CAS
2007年第3期144-148,共5页
Chinese Journal of Clinical Pharmacy
关键词
三七总皂苷
β-蜕皮激素
脂质体
凝胶剂
透皮吸收
saponins of panax notoginseng
β-ecdysone
liposomes
gel
percutaneous absorption
