摘要
建立了露水草提取物的质量标准。用薄层色谱法对其主要成分β-蜕皮激素进行鉴别；以高效液相色谱法对露水草提取物中β-蜕皮激素进行定量分析，流动相为乙腈-甲醇-水（1：2：4），流速为1mL／min，检测波长为243nm。结果薄层色谱无干扰，专属性强；β-蜕皮激素质量浓度在0．6～4．2mg／mL范围内与峰面积线性关系良好，r=0．99997（n=7），加样回收率为99．7％，RSD为0．6％（n=6）。所用方法简便、准确、重现性好，可用于露水草提取物的质量控制。
The quality standard for Extractum Cyanotis Vaga siccus was established with a TLC method for indentified beta -hydroxyecdysone in Extractum Cyanotis Vaga siccus and a HPLC method for determining simultaneously beta- hydroxyecdysone in Extractum Cyanotis Vaga siccus. The mobile phasa was made up of acetonitrile - methanol - water （ 1 ： 2 ： 4）, the flow rate was 1 mL/min, and the ultraviolet detection was set at 243 nm. The quantitative evaluation of beta - hydroxyecdysone had the linear range of 0.6 - 4.2 mg/mL （ r = 0. 999 97 ,n =7）, the average recovery was 99.7% （RSD =0. 6% ,n =6）. This method is simple and accurate with high specificity and good repeatability. It is suitable for quality control of Extractum Cyanotis Vaga siccus.
出处
《中国兽药杂志》
2009年第8期28-30,共3页
Chinese Journal of Veterinary Drug
