摘要
目的：研究苯多次吸入对小鼠的致微核作用。方法：采用静式吸入染毒的方法，苯浓度为１／８～１／６４ＬＣ５０，随机分为４组，每隔２４ｈ染毒１次，每次２ｈ，共３０ｄ；另设对照组。染毒结束后，取血作白细胞计数，取胸骨骨髓作微核计数。白细胞计数的比较用Ｆ检验，微核率的比较用率的ｕ检验。结果：白细胞计数在正常值范围，实验组与对照组差异无显著性（Ｐ＞０．０５）；但微核率，实验组比对照组明显增高（Ｐ＜０．０１），且有剂量反应关系。结论：苯长期吸入染毒可引起骨髓细胞遗传物质的损伤，且早于外周血白细胞总数的变化。
Objective:The purpose of this article is to study the micronucleus induction after repeated inhalation of benzene.Methods:Mice were divided at random into 4 experimental groups and 1 control group.Static inhalation of benzene was given to the experimental mice in concentration of 1/8～1/64 LC 50 ,every 24 h,2 h each time,for 30 d.All animals were killed at the end of the experiment.Blood and bone marrow were taken for enumeration of WBC count and micronucleus rate respectively. F test was used for enumeration of WBC count and u test for micronucleus rate.Results:The WBC counts of mice were within the normal range,with no significant difference between the test group and control group ( P >0.05).But the micronucleus rates of the experimental groups were obviously higher than that of the control group ( P <0.01) and there existed a dose response relationship within the test groups.Conclusion:Repeated inhalation of benzene may injure the genetic material of bone marrow,before it can affect the total WBC count.
出处
《蚌埠医学院学报》
CAS
1997年第1期3-4,共2页
Journal of Bengbu Medical College
关键词
苯中毒
微核
benzene
intoxication through inhalation
micronucleus
