摘要
以蝴蝶兰试管苗幼叶为外植体,以1/2MS为基本培养基,研究了不同浓度6-苄基腺嘌呤(6-BA)、腺嘌呤硫酸盐(Ad)、萘乙酸(NAA)及其组合对胚状体发生的影响,同时也探讨了蔗糖浓度及椰汁添加量对胚状体形成的影响。结果表明:细胞分裂素6-BA对胚状体的诱导效果较Ad好,NAA与二者配合使用可提高胚状体的发生率,其最佳浓度组合为6-BA4.0mg/L+Ad2.0～4.0mg/L+NAA1.0～2.0mg/L。蔗糖20～40g/L+椰汁200mL/L有利于胚状体的形成。组织学观察表明,胚状体起源于叶片气孔附近的上表皮细胞或上表皮下方的叶肉组织细胞,为单细胞起源,其发育过程与一般植物胚状体发生发育特征相似,最终发育成为类原球茎。
The leaves of Phalaenopsis were used as explants and cultured in the 1/2MS basic medium with different kinds of plant growth regulators for somatic embryos induction. The effects of different concentrations of three kinds of plant growth regulators of 6-BA, Ad, NAA and their combinations on somatic embryogenesis were studied in in vitro culture. Meanwhile, the influences of the concentrations of sucrose and coconut water on somatic embryogenesis were studied, too. The results showed that the effects of cytokinins 6- benzyladenine （6-BA） was better than adenine sulphate （Ad） in embryoid induction. The auxin A-naphthalene acetic acid （NAA） combined with given concentrations of 6-BA and Ad could effectively raise the frequency of somatic embryogenesis. The optimum plant was 6-BA 4. 0 mg/L ＋ Ad 2. 0 - 4. 0 mg/L ＋ NAA 1. growth regulator combination for somatic embryogenesis 0 -2. 0 mg/L. 20 -40 g/L sucrose and 200 mL/L coconut water added in the medium was of benefit to somatic embryogenesis. The results of histological observations indicated that somatic embryos originated from upper epidermis and mesophyll single cell nearby stoma. The course of somatic embryos development was similar to other plants. Finally, the somatic embryos became protocorm-like body.
出处
《园艺学报》
CAS
CSCD
北大核心
2007年第2期431-436,共6页
Acta Horticulturae Sinica
基金
安徽省自然科学基金项目(070411010)
安徽省科技厅重点项目(04023069)
关键词
蝴蝶兰
叶片
胚状体
组织培养
组织学观察
原球茎
Phalaenopsis
Leaf
Somatic embryo
Plant tissue culture
Histological observation
Protocorm-like body
