摘要
利用XeCl准分子紫外激光照射少根根霉孢囊孢子,研究其生物学效应。在照射功率为2.0 kV,2.5kV和2.7 kV,脉冲次数为35-135的范围内,其致死曲线在85脉冲次数处形成高峰,在110脉冲次数处形成低谷,构成一个“峰形”曲线;与紫外灯UV致死曲线有较大的差别。通过琼脂平板分离、固态发酵,从中筛选到8株脂肪酶活力提高幅度10%以上的菌株,其中以突变株LB-5的酶活力最高,达66.4 IU/g干基,较出发菌株酶活提高了55.1%。讨论了激光照射功率、脉冲次数和致死率与根霉脂肪酶活力之间的关系。
The biological effects of R. arrhizus were studied by utilizing XeCl excimer laser in condition of radiation power being 2.0 kV ,2.5 kV and 2.7 kV ,pulse number ranging between 35 - 135. A peaky lethel curve was found when flood tide was formed at 85 pulse number and low tide was formed at 110 pulse number, which was much distinctively to the lethel curve caused by UV lamp radiation. 8 strains were selected through agar fiat separation and solid state ferment. Their lipase actives were increased by 10 %. Among them, the mutation strain LB-5＇ s lipase activity reach 66.4 IU/g, increasing 55.1% than that of original strains. The connection of the laser radiation power, pulse number and lethal rate were discussed.
出处
《激光生物学报》
CAS
CSCD
2007年第2期195-199,共5页
Acta Laser Biology Sinica
基金
安徽省优秀青年科技基金(04043051)
安徽省自然科学基金(01043102)
合肥学院科学研究发展基金(05ky002zr)
