摘要
目的 探讨血清IL-13、IL-15水平及IL-15／IL-13比值在乙型病毒性肝炎发生、发展中的意义。方法 采用双抗体夹心ELISA法检测109例乙型肝炎患者血清中IL-13、IL-15水平。结果 肝炎组血清IL-13、IL-15值及IL-15／IL-13比值与对照组比较均明显升高，两组比较差异有统计学意义（P值均为0．000）。慢性重型肝炎死亡组IL-15值及IL，15／IL-13比值明显高于治疗好转组（P〈0．05）；合并细菌感染组与无细菌感染组间比较，IL-13、IL-15值及IL-15／IL-13比值均无明显差异。相关分析表明，IL-13与总胆红素、直接胆红素分别呈显著负相关（P均〈0．05）；IL-15、IL-15／IL-13与总胆红素、直接胆红素分别呈显著正相关（P均〈0．01）。结论 乙型肝炎患者存在IL-13、IL-15值及IL-15／IL-13比值的异常，存在IL-13、IL-15的失衡；通过联合检测血清IL-13、IL-15值及IL-15／IL-13比值，能较好地反映乙型肝炎患者Th1／Th2细胞激活状态，有助于乙型肝炎患者的预后判断及指导治疗。
Objective To investigate the clinical significance of serum levels of interleukin-13, 15 and IL-15/IL-13 ratio in patients with viral hepatitis B. Methods Double antibody sandwich ELISA was used to measure the serum levels of IL-13, IL-15 in 109 patients with hepatitis B. Results The serum levels of IL-13, IL-15 and IL-15/IL-13 ratio in patients with hepatitis B group were significantly higher than that in normal control group （P=0. 000）. The serum IL-15 level of IL-15/IL-13 ratio in dead group were significantly higher than that in ameliorating group with chronic severe hepatitis （P〈0.05）. Correlation analysis showed the level of IL-13, IL-15 and IL-15/IL-13 ratio were significantly negative relative to total bilirubin, and direct bilirubin, respectively. Conclusion There is an abnormal level of IL-13, IL-15 and IL-13/IL-15 ratio in patients with hepatitis B. Combined detection on the levels of serum IL-13, IL-15 and IL-15/IL-13 ratio would reflect the Th1/Th2 cellular active status effectively and be helpful to the prediction of prognosis and instruction of hepatitis B treatment.
出处
《国际检验医学杂志》
CAS
2007年第4期301-303,共3页
International Journal of Laboratory Medicine
基金
泉州市科技计划项目课题（2005215）
