摘要
目的 观察6-羟基多巴胺致帕金森病（PD）大鼠空间学习记忆能力变化及可能的发生机制。方法 6-羟基多巴胺双侧黑质致密部注射4周后进行Morris水迷宫测试；免疫荧光检测海马和前额叶皮质内血清糖皮质激素凋节蛋白激酶（SGK）表达。结果 PD大鼠在第1天水迷宫训练中潜伏期较假手术组（P〈0．05）和正常组（P〈0．05）显著延长。在探测实验中PD大鼠穿台次数[（6．08±1．88）次]，较假手术组[（8．33±0．82）次]和正常组[（9．43±2．57）次]显著减少，同时PD大鼠在站台周围游泳路程占总路程的百分比比正常组明显减少。PD大鼠海马CA1、CA3和前额叶皮质内SGK表达较对照组、假手术组显著减少。结论 PD大鼠存在空间学习记忆障碍。SGK可能参与了大鼠空间记忆障碍的发生机制。
Objective To assess the spatial cognitive ability of Parkinson＇ s disease （PD） rats, using 6- hydroxydopamine administrated in the brain, and further explore the mechanism revolved in the performance. Methods The PD rats＇ learning and memory abilities in the Morris water maze were observed four weeks after 6- hydroxydopamine injected into the compact of Substantia Nigra bilaterally. Immunofluorescence technology was used to detect the ser/glucocorticoid-inducible kinase （SGK） in the hippocampus and prefrontal cortex. Results PD rats spent more time in finding the immerged platform in MWM task in the first training day than sham group （ P =0. 031） and control group （ P=0. 019）. In the probe test, the numbers of PD rats crossing the former platform （6.08 ± 1.88 ）were significantly less than sham group （8.33 ± 0.82） and control group （9.43 ± 2.57 ） and the distance of rats swimming around the platform and total distance were significantly decreased compared with that of control group. Meanwhile, the expression of SGK in CA1 and CA3 of hippocampus and prefrontal cortex significantly decreased. Conclusion PD rats present a deficit in spatial learning and memory in water maze, and the decreased expression of SGK in the hippocampus and prefrontal cortex may be involved in the behaviors.
出处
《中国行为医学科学》
CSCD
2007年第4期292-295,共4页
Chinese Journal of Behavioral Medical Science
基金
中国博士后科学基金资助项目（20060390129）
