摘要
为了对重离子辐照致DNA损伤中的自由基作用进行评估,本文利用γ射线对添加了各种自由基清除剂(甘露醇,维生素C和茶多酚)的质粒DNA进行辐照,凝胶电泳分析的结果表明,三种自由基清除剂对DNA均有很好的保护作用。在相同自由基清除剂浓度的情况下,甘露醇比维生素C具有更强的清除自由基的作用。与重离子辐照情况相比,这几种自由基清除剂对γ射线产生的自由基的清除作用更为明显。
To evaluate the effect of free radicals on DNA damage induced by heavy ions, pUC19 plasmid DNA solution with different kinds of scavengers (Mannitol, Vitamin C and Tea Polyphenols) is irradiated by γ rays. The DNA damages are analysed by gel electrophoresis and Alpha Innotech digital imaging system. The results show that all of the three kinds of scavengers can significantly protect DNA; mannitol is more effective than Vitamin C under the same condition; and their capacity of scavenging free radicals induced by γrays is more remarkable than those induced by heavy ions.
出处
《核技术》
CAS
CSCD
北大核心
2007年第4期255-258,共4页
Nuclear Techniques
基金
国家自然科学基金(10435020)
中国原子能科学研究院院长基金
中国原子能科学研究院核物理所所长基金(12SZZ-200602)资助