摘要
目的探讨肿瘤坏死因子α(TNF-α)、白介素6(IL-6)、核因子κB(NF-κB)在酒精性肝病(ALD)患者血清中的表达及意义,研究细胞凋亡在ALD中的作用。方法采用酶联免疫吸附试验(ELISA)法检测54例ALD患者(病例组)和31例健康体检者(正常对照组)血清中的TNF-α、IL-6、NF-κB水平,推测细胞因子及凋亡在ALD发病中的作用;采用全自动生化仪检测两组血清γ谷氨酰转移酶(GGT)、天冬氨酸氨基转移酶(AST)和丙氨酸氨基转移酶(ALT)水平。结果 (1)ALD患者血清TNF-α、IL-6、NF-κB、GGT、AST、ALT水平均显著高于正常对照组,差异有统计学意义(P<0.05);(2)血清TNF-α、IL-6、GGT水平之间呈正相关(P<0.05)。结论 TNF-α、IL-6和NF-κB作为炎症因子和核转录因子在ALD的发生发展中起到重要的作用,联合检测对于判断ALD患者肝脏的炎症反应和肝细胞凋亡有一定的参考价值。
Objective To explore the expression and significance of TNF-α,IL-6,NF-κB in alcoholic liver disease（ALD） patients and the role of apoptosis in ALD.Methods Serum levels of TNF-α,IL-6,and NF-κB of 54 ALD patients and 31 healthy subjects were analyzed using enzyme-linked immunosorbent assay（ELISA） method,with the role these cytokines played in the pathogenesis of ALD.Levels of GGT,aspartate aminotransferase（AST）,and alanine aminotransferase（ALT）were measured by an automatic biochemical analyzer.Results（1）Serum levels of TNF-α,IL-6,NF-κB,GGT,AST,and ALT were significantly higher in the ALD group than in the control group（P0.05）;（2） Positive correlations existed among serum levels of TNF-α,IL-6,and GGT（P0.05）.Conclusion As inflammatory factors and nuclear transcription factors,TNF-α,IL-6,NF-κB play important roles in the pathogenesis of ALD and combination detection could provide certain references in reading the liver inflammatory responses and hepatocyte apoptosis among ALD patients.
出处
《中国全科医学》
CAS
CSCD
北大核心
2011年第21期2405-2407,共3页
Chinese General Practice
