摘要
目的:评估五倍子的矿化调节作用,了解五倍子防根龋的药理机制。方法:在pH循环模型中测试五倍子的防龋效能。以4g.L-1五倍子为模型药物,1g.L-1氟化钠和去离子水为对照,制备人根部牙体组织块,暴露于由药物处理液、酸性缓冲液、中性缓冲液组成的pH循环模型中,循环12次,每天2次。循环结束后,采用原子吸收光谱检测酸性脱矿液钙释出量,采用激光共聚焦显微镜分析脱矿效果。结果:五倍子组钙释出率降低、荧光总量减少,表明五倍子具有抑制根面牙体组织脱矿的作用,作用虽然弱于氟化物,但是差异无统计学意义。结论:在化学条件下五倍子具有抑制根部组织脱矿的作用,再次显示了五倍子具有阻止龋病发生的作用。
Objective： To evaluate the anti-demineralization efficacy of Galla Chinesis in pH cycling model for elucidating the anti-root caries mechanism. Method： Anti-demineralization efficacy evaluation of the natural medicine in the pH-cycling models was used . Sound human root blocks were pH-cycled through the treatment solution, acidic buffer and neutral buffer. The cycling times for demineralization study were 12 times, 2 times per day. The acidic buffers were retained for calcium analysis by atomic adsorption spectroscopy. The sections of blocks were analysed after pH-cycling by CLSM. Treatments were 4 g · L^-1. Galla Chinesis, 1 g · L^-1 NaF solution and distilled water. Result： Galla Chinesis was found to inhibit the demineralization in the pH cycling model. Although the effect was not as good as fluoride, there was no significant difference between the two groups. Conclusion： These data suggest that Galla Chinesis could modulate the mineralisation behaviour of root tissue in a defined chemical circumstance. These findings support the proposition that Galla Chinesis may be a promising anticaries natural medicine in the future.
出处
《中国中药杂志》
CAS
CSCD
北大核心
2007年第6期529-531,共3页
China Journal of Chinese Materia Medica
基金
国家自然科学基金资助项目(30430800
30572409)
关键词
pH循环模型
五倍子
根面龋
脱矿
pH cycling model
Galla Chinesis
root caries
demineralization
