摘要
目的:总结成肌细胞在骨骼肌收缩功能和细胞基因治疗研究中的应用。资料来源:应用计算机检索Medline及Science Direct(Elsevier),EBSCOhost,Kluwer Online等数据库2000-01/2006-10相关骨骼肌成肌细胞及其应用研究方面的文献,检索词“myoblast,contract,mitochondria,gene treatment”,限定文献语言种类为English。同时计算机检索中国期刊全文数据库2002-01/2006-10相关骨骼肌成肌细胞及其应用研究方面的文献,检索词“成肌细胞,骨骼肌收缩,基因治疗”,限定文献语言种类为中文。资料选择:对资料进行初审,选取包括成肌细胞特性、收缩研究及应用等方面的文献,开始查找全文。纳入标准:有关成肌细胞的生物学特性、在骨骼肌收缩中的应用、在基因治疗中应用方面的文献。排除标准:重复研究、个案报告、综述、Meta分析的文献。资料提炼:共检索到125篇关于成肌细胞研究的文献,最终纳入35篇符合标准的文献。资料综合:成肌细胞是肌组织的前体细胞,易培养、可塑性强,具有骨骼肌的许多重要生物特性,且细胞能够融合进在体骨骼肌组织中,因此在骨骼肌收缩运动引起的细胞离子浓度、线粒体功能和肌型变化等的研究以及细胞移植治疗骨骼肌肌病和修复心肌组织的研究上都有着广泛的应用。本文综述近年来成肌细胞的生物学特性和骨骼肌收缩功能及基因治疗应用等方面所取得的相关进展。结论:成肌细胞可以为骨骼肌在收缩功能上的研究提供一个很好的研究平台,其在基因治疗研究中的应用也为肌组织工程的研究奠定了基础。
OBJECTIVE： To summarize the application of myoblast in skeletal muscle contraction and gene treatment research.
DATA SOURCES： A computer-based online search of Medline and Science Direct （Elsevier）, EBSCOhost, Kluwer Online was undertaken to identify the literature relevant to myoblast of skeletal muscle and its application research published in English from January 2000 to October 2006 with the keywords of ＂myoblast, contract, mitochondria, gene treatment＇. Meanwhile, we searched China Journal Full-text Database for Chinese articles about myoblast and its application research published from January 2002 to October 2006 with the keywords of ＂myoblast, contraction, gene treatment＂ in Chinese.
STUDY SELECTION： The data were selected primarily to choose those about the characteristics and contraction of myoblast and the full texts were looked over. Inclusive criteria： Literature about the biological characteristics of myoblast, the application in skeletal muscle contraction and gene treatment. Exclusive criteria： Repeated researches, case reports, reviews or Meta analysis.
DATA EXTRACTION： A total of 125 articles about myoblast were obtained, and thirty-five representative ones were selected.
DATA SYNTHESIS： Myoblast as the precursor call of muscle tissues is easy to culture and plastic; it possesses many important biological characteristics of skeletal muscle, and could integrate into the skeletal muscle tissue. Therefore, myoblast is widely used in the research of the changes of call ion concentration, mitochondria function and muscle types induced by skeletal muscle contraction and gene treatment for muscle disease and repairing cardiac muscle. In this paper, we reviewed the progress in the biological characteristics of myoblast, skeletal muscle contraction function and gene therapy application.
CONCLUSION： Myoblast provides a research platform for skeletal muscle contraction function, which also lays a foundation for the muscle tissue engineering research in gene treatment.
出处
《中国组织工程研究与临床康复》
CAS
CSCD
北大核心
2007年第10期1892-1894,1899,共4页
Journal of Clinical Rehabilitative Tissue Engineering Research
