摘要
目的探讨大鼠肌源性干细胞体外原代培养的方法,为临床应用自体肌源性干细胞注射治疗压力性尿失禁提供实验依据。方法采用胶原酶和胰蛋白酶等分离大鼠骨骼肌细胞,应用差速贴壁法进行纯化获取肌源性干细胞,在倒置显微镜下观察干细胞的形态,通过免疫组织化学法进行鉴定。结果通过差速贴壁法可获取高纯度的肌源性干细胞,免疫组织化学技术可鉴定其肌源性和干细胞特性。结论肌原性干细胞可通过体外原代培养获取,适用于组织工程和基因治疗,临床应用有待进一步研究。
Objective To establish the methods for culture and identification of rat skeletal muscle derived stem cells in vitro. Methods Rat MDSC were isolated from gatrocnemius muscle biopsies of normal 4-6 week-old-Sprague-Dawlay rats and purified by different adhesion time. CeLls that took approximately 5-6 days to adhere to the flasks were MDSC. The cells were observed by invert microscope and indentified with cellular immunochemieal stain. Results At least 90 % MDSC were harvest by the prelate technique and these cells were Sca- 1 ＋ and desmin ＋ by immunohistochemical staining. Conclusion It is easy to primary culture of MDSC in vitro and these cells can be used to tissue engineering and transgene therapy.
出处
《中华实验外科杂志》
CAS
CSCD
北大核心
2006年第3期334-335,共2页
Chinese Journal of Experimental Surgery
关键词
肌源性干细胞体
原代培养
鉴定
Muscle-derived stem cell
Primary culture
Identification
