摘要
以6个品种大豆体细胞胚为受体,以Bt基因为目的基因,应用根癌农杆菌介导法对大豆进行了遗传转化,经体细胞胚萌发、生根及壮苗培养和卡那霉素选择培养,获得了完整的抗性再生苗,经PCR、PCR-Southern和点杂交分析鉴定,初步证明外源基因Bt已整合到大豆的基因组中。
Somatic embryos of 6 different soybean were transformed by Agrobacterium tumefaciens. The resistant plants to kanamycin were obtained. 5 positive plants were obtained among them by PCR. These positive plantlets were further analyzed by dot blotting and PCR-Southern blotting. The results demonstrated that the target gene had been transformed into the soybean genome.
出处
《大豆科学》
CAS
CSCD
北大核心
2007年第1期103-106,共4页
Soybean Science
基金
"国家植物转基因技术研究开发与中试基地建设"专项课题(J99-B-001)资助
关键词
大豆
体细胞胚
BT基因
遗传转化
Soybean
Somatic embryos
Bt gene
Genetic transformation
