摘要
目的:研究食管癌组织中PTEN和MGMT基因蛋白的表达及两者的关系。方法:采用免疫酶标和免疫荧光技术分别在光镜和激光扫描共聚焦显微镜(CLSM)下检测105例食管癌组织中的PTEN和MGMT基因蛋白的表达。结果:PTEN蛋白在食管高、中和低分化鳞癌组织中的阳性率分别为63.64%、30.43%和27.27%。高分化鳞癌与中分化鳞癌阳性率比较,差异有统计学意义,u=169.00,P<0.05。高分化鳞癌组织的PTEN蛋白表达的量(88195.69±50794.95)明显高于中分化鳞癌组织(28100.27±35988.47),t=3.053,P<0.01。MGMT蛋白在食管鳞癌、腺样结构癌和小细胞癌组织中的阳性表达率分别为76.79%、90.00%和11.11%,小细胞癌MGMT蛋白表达与鳞状细胞癌及腺样结构食管癌的表达差异有统计学意义,u=82.00,P<0.01;u=19.00,P<0.01。食管癌组织中PTEN与MGMT蛋白的阳性表达呈正相关,r=0.574,P<0.01。结论:PTEN蛋白表达与食管鳞癌的分化程度有关,而MGMT蛋白表达则与食管癌的组织学类型有密切关系,两者可作为食管癌预后判断的参考指标。
OBJECTIVE： To investigate the expressions and their relationship of PTEN and MGMT gene proteins of the esophageal carcinoma. METHODS： Expressions of PTEN and MGMT genes in 105 cases of esophageal carcinoma were detected under the light microscope and confocal laser scanning microscope by the immunoenzyme and immunofluorescence techniques. RESULTS： The positive rates of PTEN in the well differentiated esophageal squamous cell carcinoma （ESCC）, moderately differentiated ESCC and poor differentiated ESCC were 63.64 %, 30.43 % and 27.27 %, respectively. The positive rates were compared between the well differentiated ESCC and moderately differentiated ESCC, and the difference was significant, u= 169.00, P〈0.05. The expression of PTEN of the well differentiated ESCC （88 195.69±50 794. 95） was significantly higher than that of moderately differentiated ESCC （28 100.27 ± 35 988.47）, t=3.053, P〈0.01. The positive rates of MGMT in ESCC, esophageal carcinoma with adenoid structure and small cell carcinoma were 76.79%, 90.00% and 11.11% ,respectively. Significant differences were observed in the expression of MGMT between small cell carcinoma and esophageal squamous cell carcinoma, and between small cell carcinoma and esophageal carcinoma with adenoid structure （u=82.00,P〈0.01;u=19.00,P〈0.01）. Apositive correlation was observed between the expressions of PTEN and MGMT （r= 0. 574,P〈0. 01）. CONCLUSION： The expressions of PTEN and MGMT are correlated with the differentiation of ESCC and histological type of the esophageal carcinoma, respectively, which may be used as important indexes to evaluate the prognosis of esophageal carcinoma.
出处
《中华肿瘤防治杂志》
CAS
2007年第1期31-34,共4页
Chinese Journal of Cancer Prevention and Treatment
关键词
癌
鳞状细胞/代谢
食管肿瘤/代谢
免疫组织化学
食管肿瘤/病理学
carcinoma, squamous cell/metabolism
esophageal neoplasms/metabolism
immunohistochemistry
esophageal neoplasms/patholgy
