摘要
目的:探讨O6-甲基鸟嘌呤-DNA甲基转移酶(O6-methylguanine-DNA methyltransferase,MG-MT)在甲状腺癌组织中的表达及其临床意义。方法:用免疫组织化学SP法检测61例甲状腺癌、21例甲状腺良性腺瘤、15例桥本氏甲状腺炎、8例结节性甲状腺肿和12例癌旁正常组织中MGMT蛋白的表达,并结合临床病理因素进行分析。结果:MGMT在甲状腺癌组织中的表达与正常组织中的表达差异有统计学意义(P<0.05)。MGMT表达从正常组织(16.67%,10/12)、结节性甲状腺肿(25.00%,2/8)、桥本氏甲状腺炎(60.00%,9/15)、甲状腺腺瘤(52.38%,11/21)到甲状腺癌(60.66%,38/61)中表达水平基本呈上升趋势。在甲状腺乳头状癌与甲状腺滤泡癌的表达差异有统计学意义(P<0.05),表达水平随甲状腺癌恶性程度的增加而降低,分别为乳头状癌(72.22%,26/36)、滤泡癌(50.00%,8/16)。MGMT在性别、年龄及民族组中表达差异均无统计学意义(P>0.05)。结论:MGMT高表达现象可能与甲状腺癌恶性程度有关,可成为候选的临床分子诊断指标。甲状腺癌组织的MGMT蛋白在性别、年龄和民族组表达均无差异,有望成为通用的临床检测指标。
Objective To study the expression of O6-methylguanine-DNA methyltransferase （MGMT） and its clinicopathological significance in thyroid cancer.Methods Immunohistochemistry was used to determine the expression of MGMT in 61 thyroid cancer tissues, 21 thyroid adenomas, 15 Hashimoto’s thyroiditis, 8 nodular goiter, and 12 peri-tumor tissues. Results There was statistic difference in the expression of MGMT between the normal tissues and thyroid cancers （P0.05）. Expression of MGMT increased from the normal tissue （16.67%, 10/12）, nodular goiter （25.00%, 2/8）, Hashimoto’s thyroiditis （60.00%, 9/15）, and thyroid adenoma （52.38%, 11/21）to thyroid cancer （60.66%, 38/61）. Expression of MGMT in papillary thyroid cancer （PTC） and follicular thyroid cancer （FTC） had significant difference （P0.05）, and the expression level of MGMT decreased with the malignancy of thyroid cancer, such as in PTC （72.22%, 26/36）, and FTC （50.00%, 8/16）. There was no statistic difference in MGMT expression in sex, age, and nationality （P0.05）.Conclusion High expression of MGMT might be related to the malignancy of thyroid cancer, which may be one of the diagnosis indexes for thyroid cancer. It will be a common clinical index in diagnosing thyroid cancer since there is no difference in MGMT expression among sexes, ages, and nationalities.
出处
《中南大学学报（医学版）》
CAS
CSCD
北大核心
2010年第12期1219-1224,共6页
Journal of Central South University ：Medical Science
基金
supported by the National Natural Science Foundation of China (30860275)
the Key Laboratory Fund of Xin-jiang Autonomous Region(XJDX0208 -2008 -07)
