摘要
通过摇瓶液体培养试验,对皱柄白马鞍菌液体深层培养中培养基成分、pH值、装液量及摇床转速、培养时间等对菌丝体生长的影响进行了对比研究。结果表明,适宜的培养条件为:培养基含小麦粉20 g/L、硫酸氨2g/L、MgSO40.5 g/L、KH2PO40.46 g/L、K2HPO41 g/L,最适pH 5.5-6.5,装液量300-400 mL/L,接种量5 mL培养菌液,温度26-28℃;摇床转速100 r/min,培养时间7 d。适宜条件下最大菌丝生物量可达5.867 g/L。
The culture conditions of Helvella crispa Fr. in shake flask liquid culture were investigated in the experiment. Results showed that the optimal medium of liquid submerged culture was as follows： wheat flour 20 g/L,HN4SO4 2g/L,MgSO4 0.5 g/L,KH2PO4 0.46 g/L,K2HPO4 1.0 g/L;when the medium capacity was 300-400 mL/L,and the seed （about 5 mL） was inoculated,the shake flask culture experiment was carried out at 26-28℃,pH 5.5-6. 5 in a shake incubator with a rotation rate 100 r/mn for 7 days. The maximum mycelial biomass was as high as 5. 867 g/L.
出处
《西北农林科技大学学报（自然科学版）》
CSCD
北大核心
2007年第3期223-226,共4页
Journal of Northwest A&F University(Natural Science Edition)
基金
教育部优秀博士论文基金资助项目(200057)
