摘要
采用氯仿:异戊醇一次抽提法提取茶树基因组DNA,再经纯化,结果表明,此法提取的DNA其OD260/OD280值在1.70～1.85之间,相对分子量≥23kb,且能被EcoRI酶解完全,并能获得清晰PCR图谱和AFLP指纹图谱。
Genomic DNA was isolated once from tea plant by chloroform and isoamyl alcohol. The results showed that the values of OD260/OD280 for DNA samples obtained by this method are between 1.70 and 1.85 respectively with the molecular weight of DNA higher than 23kb. The DNAs have been completely digested by EcoR I. Amplified figures in the PCR, and AFLP fingerprinting figures are all obtained.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2007年第2期193-196,共4页
Food Science
基金
广东省农科院资助项目(SR0273)
关键词
茶树
AFLP
DNA提取方法
tea plant
AFLP（amplified fragment length polymorphism）
DNA extraction
