摘要
采用AFLP分子标记技术,利用筛选出6对多态性较强的引物组合,构建了白花蛇舌草25份种质的AFLP指纹图谱.6对引物组合共扩增出888条谱带,其中多态带661条,占73%.聚类分析结果表明,来源地相同的白花蛇舌草居群多表现出较为密切的亲缘关系,不同来源地的白花蛇舌草居群间遗传差异相对较大,其中海南白花蛇舌草独自呈类,与其它省份的亲缘关系较远.
AFLP fingerprints of Hedyotis diffusa were established by AFLP technique and six primer pairs.There were 888 bands,of which 661 were polymorphic,accounting for 73%.The cluster analysis indicates: most of the population in the same area have the closer relationship,the heredity difference of the population from different areas is relatively big.And the population from Hainan being a kind alone is far with other population in relationship.
出处
《河南农业大学学报》
CAS
CSCD
北大核心
2010年第6期635-638,共4页
Journal of Henan Agricultural University
关键词
白花蛇舌草
种质资源
遗传多样性
聚类分析
Hedyotis diffusa
germplasm
genetic diversity
AFLP
cluster analysis