摘要
目的:探讨黑大豆种皮花色苷(BSCA)的体外抗氧化作用。方法:采用化学模拟体系测定其总抗氧化能力、还原力以及清除DPPH.能力;体外动物实验研究了BSCA对37℃孵育后肝匀浆中谷胱甘肽过氧化物酶(GSH-Px)活力、以及37℃孵育后H2O2诱导的脂质过氧化产物MDA的生成和红细胞溶血的影响。结果:BSCA(390ng/ml)的氧自由基清除活性(ORAC)值为4.63U/ml,其总抗氧化能力强于同浓度的维生素C,其还原力与浓度呈显著正相关(P<0.01),对DPPH.具有较强的清除能力,且可增强GSH-Px活力,抑制MDA的产生和红细胞(RBC)溶血。结论:认为BSCA具有较强的抗氧化作用。
Objective： To investigate the antioxidant effects of black soybean coat anthocyanins （BSCA）. Methods： The capacity of total antioxidant and reduction and DPPH. scavenging ability of BSCA were measured by the chemical simulation.The effect of BSCA on GSH-Px activity and MDA production of liver tissues and hemolysis of RBC induced by H2O2 were analyzed in vitro. Results： The oxygen-radical absorbance capacity（ORAC） value of BSCA （390ng/ml） was 4.63U/ml, the positive correlation （P〈0.01） existed between reductive capacity and concentration ,BSCA could scavenge DPPH., enhance the activity of GSH-Px,inhibit production of MDA and hemolysis of RBC. Conclusion： BSCA had antioxidant effect in vitro.
出处
《营养学报》
CAS
CSCD
北大核心
2007年第1期54-57,共4页
Acta Nutrimenta Sinica
基金
国家自然科学基金(No.30200171)
关键词
黑大豆种皮
花色苷
抗氧化
体外
black soybean coat
anthocyanin
anti-oxidative effect
in vitro
