摘要
目的：确定用于分离、纯化及保种所用的固体培养基中优化的琼脂浓度和利于微藻生长并抑菌的抗生素浓度。方法：设计固体培养基中的琼脂浓度以及培养液中抗生素的种类和浓度，定时测定培养体系中三种微藻的生物量及细菌菌落随培养时间的变化。结果：利于固体培养基上三种微藻生长的琼脂浓度均为0．6％-0．8％；牟氏角毛藻用2001U／ml的庆大霉素和2001U／ml的青霉素联合作用，球等鞭金藻8701和盐藻分别用3001U／ml和400IU／ml的青霉素时可保证微藻较好生长。结论：不同微藻生长所需的抗生素浓度不同，抗生素对微藻生长的影响不完全取决于抑菌作用，青霉素促进球等鞭金藻8701生长主要是产生促生长因子，庆大霉素抑制生长可能是产生抑制因子。
Objective：The optical agar concentration in solid culture tor separating and purifying and preserving microalgae was determined and the optical antibiotics concentration for facilitating the growth of microalgae and restraining that of bacteria was also done. Methods： The agar concentration in solid culture was designed and the species and concentration of antibiotics in culture medium were also designed, the changes of the biomass of three micmalgae and bacterial amount with culture time were determined at a settled period. Results： 0.6%-0.8% of agar concentration was favorable for the developing of three kinds of microalgae on the solid culture; the optimal antibiotics for Chaetoceros muelleri Lemmermann was the mixture of 200 IU/ml gentamycin and 200 IU/ml penicillin, while 300 IU/ml penicillin for Isochrysis galbana 8701 and 400 IU/ml penicillin for Dunaliellas sp. was preferable , respectively. Conclusion： The different microalga needs the different concentration and species of antibiotics for growth, and the effects of gentamycin and penicillin on the growth of microalgae were not thoroughly inhibiting bacteria, penicillin accelerated the growth of Isochrysis galbana 8701 because of prt）ducing growth factors, while gentamycin restrained the growth of micmalgae because of producing inhibition factors.
出处
《生物技术》
CAS
CSCD
2006年第6期75-77,共3页
Biotechnology
关键词
海洋微藻
固体培养基
抗生素
琼脂浓度
marine microalgae
solid culture
antibiotics
agar concentration
