摘要
对耐低温品系球等鞭金藻 870 1在低温下与活性氧清除和伤害有关的几个生理生化指标进行了研究 ,探讨了其耐低温机理 ,并与该藻种的低温敏感品系球等鞭金藻 30 11进行了比较。结果表明 ,⑴球等鞭金藻 870 1在低温下 ( 8℃ )培养一周 ,其超氧化物歧化酶 (SOD)活性略有下降 ,但仍保持极高的酶活性 ,因此能够及时清除低温胁迫产生的过量活性氧 ,使其细胞内保持较低的膜脂过氧化水平和较低的电解质外渗率 ;⑵球等鞭金藻30 11在低温下胁迫一周 ,其细胞内的膜脂过氧化水平和电解质外渗率大大高于球等鞭金藻 870 1,这与其SOD活性较低 ,并且在低温下其活性大幅度下降有关 ;⑶与SOD相比 ,两种不同品系球等鞭金藻的过氧化物酶(POD)对减轻其低温伤害、提高其耐低温能力 。
This paper reports several physiological and biochemical changes related to active oxygen injure and scavenging effect in a low temperature resistant strain Isochrysis galbana Parke 8701 under low temperature stress and investigates its low temperature tolerant mechanism in comparison with a low temperature sensitive strain I. galbana Parke 3011.The experimental results showed that:⑴ the activity of superoxide dismutase (SOD) in I. galbana Parke 8701 decreased slightly when cultured at low temperature (8℃) for a week, but still kept extremely high enzymatic activity, so it could scavenge the extra active oxygen rapidly under low temperature stress and keep a membrane lipid peroxidation in relatively lower level as well as a relatively low electrolytic leakage; ⑵the membrane lipid peroxidation level and electrolytic leakage in I. galbana Parke 3011 were significantly higher than in I. galbana Parke 8701 when stressed under low temperature for a week. This was due to the relatively low SOD activity in the former and its activity rapidly declined; ⑶in comparison with SOD, peroxidase (POD) did not play an important role in relieving the low temperature injury and raising the capacity of low temperature tolerance in these two strains of I. galbana Parke.
出处
《水产学报》
CAS
CSCD
北大核心
2001年第1期20-25,共6页
Journal of Fisheries of China
基金
国家自然科学基金资助项目! (39870 14 6 )
山东省自然科学基金资助项目! (Y95D0 14 4 )
