摘要
目的:测定通解口服液中大黄素的含量。方法:采用高效液相色谱法,色谱柱为Lichrospher C18;流动相为甲醇-0.1%磷酸(85∶15,v/v);流速为1.0mL.min-1;检测波长为254nm。结果:精密度和稳定性良好,大黄素在0.001 6-0.0160μg进样范围内呈良好线性关系,r=0.999 7,平均回收率为97.17%,RSD为0.5%(n=5)。结论:本方法简便、专属、重现性好,可有效控制通解口服液的质量。
OBJECTIVE To determine the content of emodin in of Tongjie oral solution. METHODS HPLC was used. the stationary phase was Lichrospher C18; the mobile phase consisted of methanol 0. 1% phosphoric acid（85 ： 15, v/v） ; with the flow rate of 1.0 mL, min^-1 ; and detection wavelength at 254 nm. RESULTS Linear range of determination of emodin was 0. 001 6 - 0. 0160μg（r= 11. 9997）. The mean recovery was 97. 17% for emodin, RSD was 0. 5%. CONCLUSION The method is convenient,selective and reproducible for determination of paeoniflorin and terandrine in Tongjie oral solution.
出处
《中国医院药学杂志》
CAS
CSCD
北大核心
2006年第12期1483-1485,共3页
Chinese Journal of Hospital Pharmacy
