摘要
目的:观察海洛因依赖SD大鼠杏仁核神经元自发放电及伤害性刺激对神经元自发放电的影响。方法:实验于2004-12/2005-02在贵阳医学院生理教研室完成。选择健康SD大鼠45只,随机分为海洛因依赖组穴n=25雪和对照组穴n=20雪。海洛因依赖组按剂量穴13mg/kg雪逐日递增原则,2次/d,连续9d皮下注射海洛因,首日剂量3mg/kg。对照组以同样方法注射等量生理盐水。第10天用5mg/kg纳洛酮腹腔注射催促戒断,确定海洛因依赖模型的建立成瘾后的大鼠从第10天起,给予海洛因维持剂量27mg/kg,1次/d。对照组同样方法注射等量生理盐水。给药12d后用玻璃微电极细胞外记录两组大鼠杏仁核神经元自发放电及鼠尾伤害性刺激对自发放电的影响。结果:纳入大鼠45只,实验组在建立模型过程中有2只死亡,另外随机选取大鼠补充,最终进入结果分析大鼠保持为45只。①海洛因依赖组记录到114个单位神经元放电,对照组记录到80个。②两组大鼠杏仁核神经元可观察到单个不匀、束簇状和混合型3种放电形式熏海洛因依赖组和对照组杏仁核神经元自发放电均以单个不匀为主,对照组中束簇状穴7.5%雪和混合型穴13.8%雪放电所占比例高于海洛因依赖组穴1.8%和7.0%雪,组间差异有显著性意义(P<0.05)。③海洛因依赖组大鼠杏仁核自发放电以低频为主(57.9%),而对照组则以低频(46.3%)和中频(47.5%)为主(P<0.05)。④给予伤害性刺激后,大鼠杏仁核神经元自发放电表现为激活、抑制和无影响3种效应。实验组大鼠杏仁核神经元中频放电以抑制(45%)和无影响(40%)为主,而对照组以无影响为主(70%)。组间差异有显著性意义穴P<0.05雪。⑤伤害性刺激后两组大鼠杏仁核神经元低频放电无变化。结论押海洛因对大鼠杏仁核神经元自发放电的形式和频率产生影响,海洛因对伤害性刺激后大鼠杏仁核神经元中频放电产生影响。
AIM： To observe the spontaneous unit discharge of the amygdoid nucleus without or with noxious stimulation in heroin-dependent rats.
METHODS： The experiment was carried out at the Staff Room of Physiology, Guiyang Medical College from December 2004 to February 2005. Forty-five healthy SD rats were randomized into heroin-dependence group （n=25） and control group （n=20）. Heroin was injected subcutaneously in the dependent rats twice a day continuously for 9 days according to the principle of daily increasing dose 13 mg/kg, while saline of equal dose was administrated subcutaneously in the control rats. On the 10^th day, the withdrawal signs abstained by intraperitoneal injection of 5 mg/kg naloxone was evaluated to ensure the establishment of heroin-dependence models. From the 10^th day, the addicted rats were injected with 27 mg/kg heroin, once per day, while the saline of equal volume were given in the contral group. The spontaneous unit discharge of amygdoid nucleus and the effects of noxious stimulation in rat tails were observed in both groups by the extracellular single-unit recording with glass microelectrodes after 12-day administration.
RESULTS： Totally 45 rats were involved in the result analysis, and 2 rats died in the model establishment but another 2 ones were recruited randomly in time. ① There were 114 units discharging in heroin-dependence group and 80 units discharging in control group. ② The spontaneous discharges of amygdoid nucleus consisted of single, irregular form, cluster form and complex form in both groups, but single and irregular form was dominant. The percentage of cluster and complex （7.5%, 13.8%） discharges in control group were higher than those in heroin-dependent group, with the significant （1.8%, 7.0%, P 〈 0.05）. ③ The spontaneous discharges in heroin-dependence group were dominantly low frequency （57.9%）, whereas low frequency （46.3%） and middle frequency （47.5%） were the main frequencies in control group （P 〈 0.05）. ④ After noxious stimulation, the spontaneous discharges were identified as activated, inhibitory and unaffected effects. Most of the middle frequency discharges of amygdoid nucleus in control group were unaffected （70%）, whereas in heroin-dependence group, the ratio Of the inhibitory and unaffected neurons was 45% and 40% respectively, with the significant difference （P 〈 0.05）. ⑤ There was no change in the low frequency discharges of amygdoid nucleus after noxious stimulation in all the rats.
CONCLUSION： Heroin makes great impact on the form and frequency of spontaneous unit discharges in the dependent rats as well as the middle frequency discharge of rats after noxious stimulation.
出处
《中国临床康复》
CSCD
北大核心
2006年第46期89-92,共4页
Chinese Journal of Clinical Rehabilitation
