摘要
目的观察和评价针刺及青藤碱(sinomenine)治疗对海洛因成瘾大鼠戒断症状及其杏仁核(amygdala)自发放电及突触素(synaptophysin)表达的影响。方法75只SD大鼠随机均分5组:对照组、成瘾组、针刺组、青藤碱组和联合治疗组。皮下注射海洛因建立成瘾模型大鼠。3个治疗组分别给予针刺穴位(内关、百会、足三里)、青藤碱肌注及两者联合治疗。评估各组大鼠诱导戒断症状,用电生理技术观察5组大鼠杏仁核自发放电,免疫组织化学技术检测杏仁核突触素表达。结果给予纳洛酮后,大鼠出现典型诱导戒断症状(P<0.01),造模成功;给予针刺及青藤碱治疗后,其戒断症状明显减轻(P<0.01)。杏仁核自发放电结果显示:相对于对照组,海洛因成瘾大鼠自发放电低频放电和单个不匀放电比例增多,束簇状和混合型放电比例明显减少(P<0.05),而给予青藤碱或联合治疗的成瘾大鼠放电形式得以逆转(P<0.05),接近对照组水平。免疫组化结果显示,相对于对照组,成瘾组大鼠杏仁核突触素表达增多(P<0.01),给予针刺、青藤碱及联合治疗的大鼠杏仁核突触素的表达明显减少(P<0.01)。结论针刺及青藤碱治疗可以减轻海洛因成瘾大鼠的戒断症状,一定程度地纠正成瘾大鼠杏仁核自发放电频率和形式的异常,降低海洛因成瘾大鼠杏仁核突触素的表达,且二者联合应用效果更好。
Aim To explore the effects of acupuncture and sinomenine on the withdrawal symptoms,on the spontaneous discharge and synaptophysin expression of amygdala of heroin-addicted rats.Methods 75 SD rats were randomly and equally divided into 5 groups consisted of control,heroin-addicted,acupuncture,sinomenine and combined treatment group.The model of heroin-addicted rats were established with heroin subcutaneous injection,which in acupuncture,sinomenine and combined group were subsequently punctured at 3 acupoint（Neiguan,Baihui and Zusanli）,intramuscular injected with sinomenine and treated with combined methods（acupuncture and sinomenine）respectively.The withdrawal symptoms of rats in different group were evaluated,and then the spontaneous discharges and synaptophysin expression of amygdala were detected with electrophysiological and immunohistochemical technology respectively.Results The heroin-addicted rats were successfully established,being suggested by typical withdrawal symptoms induced by naloxone（P〈0.01 vs control）,which were alleviated in the 3 treatment groups（acupuncture,sinomenine and combined ） with varying degree（P〈0.01 vs heroin group）.The electrophysiological results suggest that the forms of low-frequency and single irregular discharges in amygdala were increase,bunchy/cluster and complex discharges were decreased（P〈0.05 vs control）,which were reversed apparently in sinomenine and combined treatment groups（P〈0.05 vs heroin group）,closed to control.The immunohistochemical results show that expression of synaptophysin in amygdala was increased obviously（P〈0.01 vs control）,which was decreased in the 3 treatment groups（acupuncture,sinomenine and combined）,P〈0.01 vs addicted group.Conclusion Treatments of acupuncture and sinomenine administration can alleviate withdrawal symptoms of heroin-addicted,and rectify the abnormal spontaneous discharges and expression of synaptophysin in amygdala of heroin-addicted rats for certain degree,especially with combined treatments.
出处
《中国药理学通报》
CAS
CSCD
北大核心
2010年第4期505-508,共4页
Chinese Pharmacological Bulletin
基金
贵州省教育厅自然科学类科研资助项目(No黔教科2005212)
关键词
青藤碱
针刺
海洛因成瘾
戒断症状
杏仁核
突触素
自发放电
sinomenine
acupuncture
heroin-addiction
withdrawal symptom
amygdale
synaptophysin
spontaneous discharge
