摘要
目的观察冰蜜生肌膏对兔皮肤缺损修复的促进作用。方法家兔随机分为三组,分别用1∶5比例配制的无菌冰蜜生肌膏、百多邦软膏均匀涂抹伤口,空白组单用生理盐水纱布覆盖,常规包扎伤口。各组动物每日各用冰蜜生肌膏、百多邦软膏、生理盐水纱布换药1次。术后第1、2、3、4周各处死3只动物,并切取新生皮肤标本,用福尔马林固定后送至病理室处理,对标本进行免疫组化染色,通过图象分析系统进行细胞计数和测定平均光密度值。结果通过动物造模实验,对第1、2、3、4周分别切取的新生皮肤标本进行免疫组化染色及细胞计数、平均光密度值测定比较,冰蜜生肌膏组与空白组比较有显著性差异,与百多邦组比亦有显著性差异。结论冰蜜生肌膏能促进成纤维细胞的生长,从而促进皮肤缺损的修复。
Objective:To observe the influence of Borneol and Honey Ointment(BHO,冰蜜生肌膏) on promoting the skin defect of rabbits to repair through the animal experiments.Methods:To use the aseptic BHO(1∶5),Bactroban(Mupirocin ointment) to daub the wounds respectively on the mould animals,and the group of normal saline(NS) simply used the NS gauze to mantle,then binded up the wound.Every day respectively used thedifferent medicine to replace the gauze.Every week killed 3 rabbits and severed specimen of the new-born skin,used formalin to fix then take to pathology room to handle.After be immunohistochemistry stained,used automatic analyticl system of the picture to count the numbers of fibroblast and measure the numbers of average light density.Results:Compared with the group of NS and that of bactroban,the numbers of fibroblast and the numbers of average light density of BHO group were quite different.Conclusion:BHO could promote fibroblast to grow and thereby promote the skin defect to repair.
出处
《中国中医急症》
2006年第12期1381-1381,1389,共2页
Journal of Emergency in Traditional Chinese Medicine
关键词
冰蜜生肌膏
成纤维细胞
皮肤缺损
免疫组化
Borneol and Honey Ointment(冰蜜生肌膏)
fibroblast
skin defect
immunohistochemistry
