摘要
目的:调查湖北汉族人群人类白细胞抗原(HLA)-DRB1基因多态性,获得完整准确的遗传学数据。方法:应用序列特异性引物聚合酶链反应(PCR-SSP)方法对213名随机选取无亲缘关系的湖北籍汉族健康体检者或健康献血员进行HLA-PDRB1基因型检测。结果:在213名正常个体中,发现基因频率(GF)较高的有DRB1*1501-1502(GF=11.0%)、*1101-1105(GF=8.5%)、*0901(GF=6.8%)、*1301-1302(GF=6.6%)、DRB4*0101(GF=19.0%)、DRB5*0101(GF=16.9%),低频率等位基因是DRB1*04、*1001和*0101-0103。结论:从基因水平分析了HLA-DRB1基因在湖北汉族群体中的分布特征,提供了一套比较完整准确的DRB1基因频率,为人类学和疾病相关性等研究提供了重要的参考数据。
Objective： To investigate at the DNA level the polymorphism of HLA-DRB1 gene in Han population in Hubei province of China. Methods： A total of 213 healthy examination persons or healthy donors without blood relationship in Han nationality of Hubei province were selected and analyzed by using the polymerase chain reaction/sequence specific primer （PCR/SSP） to test their HLA-DRB1 allele gene frequencies（GF）. Results： The most frequent alleles were DRB1 ＊ 1501- 1502（GF= 11. 0%）, ＊ 1101-1105 （GF= 8. 5%）, 6.6%）, DRB4 ＊ 0101（GF=19.0%）, DRB5 ＊ 0101 0901（GF=6. 8%）, ＊ 1301-1302（GF= （GF= 16.9%） and the lowest gene frequencies were DRB1 ＊ 04, ＊ 1001 and ＊ 0101-0103. Their gene frequencies and allelic distributions were compared to those observed in other ethnic populations. Conclusion： The results showed the characteristic of HLA-PDRB1 distribution, and provided more comprehensive and accurate gene data that may serve as normal reference values for the population in Hubei province.
出处
《武汉大学学报（医学版）》
CAS
2006年第6期748-752,共5页
Medical Journal of Wuhan University
