摘要
目的分析兰州地区汉族人群HLA-A、B和DRB1位点等位基因多态性特点。方法采用序列特异性引物聚合酶链反应技术对兰州地区200名健康无血缘关系的汉族个体HLA-A、B和DRB1基因座进行分型,并与西北、北方和南方汉族、西北回族、维吾尔族和藏族人群进行比较。结果兰州汉族人群中HLA-A基因座共检出14个等位基因,以A02,A11,A24,A33,A30,A01和A31基因最常见;HLA-B基因座共检出32个等位基因,以B40,B15,B46,B13,B51,B60,B58和B44基因最为常见;HLA-DRB1基因座共检出13个等位基因,最多见的基因依次为DRB109,DRB115,DRB112,DRB104,DRB111,DRB107,DRB108和DRB114,接近北方汉族而与南方汉族有差异,与西北回族无明显差异,但与西北维吾尔族和藏族差异有统计学意义。结论兰州地区汉族人群HLA-A、B和DRB1位点等位基因多态性与南、北汉族人群存在不同程度的差异,与西北维吾尔族和藏族差异显著。
Objective To investigate the HLA-A, B and DRB1 allele polymorphism of the Han race population in Lanzhou area. Methods Polymerase chain reaction-sequence specific primer was used to detect HLA-A, B and DRB1 alleles in 200 unrelated heathy Han individuals from Lanzhou region, Northwest China, and the results were compared with those of Han populations in North, South and Northwest China, and Hui, Uigur and Tibetan population in China. Results Fourteen of alleles were detected and identified for HLA-A; 32 for HLA-B; and 13 for HLA-DRB1. HLA- A＊01, A＊02,A＊11,A＊24, A＊30, A＊31, A＊33; HLA- B＊3, B＊15, B＊40, B＊44, B＊46, B＊ 51, B＊58, B＊60; HLA-DRB1＊04,. DRB1＊07, DRB1＊08, DRB1＊09, DRB1＊11, DRB1＊12, DRB1＊14 and DRB1＊15 were the most common alleles. The frequencies of HLA-A, B and DRB1 genes of Lanzhou Han race were close to that of North China Hans and Hui population in Northwest China, and a little different to that of South China Hans. The HLA-DRB1 alleles were significantly different to those of Uigur and Tibetan race population of China. Conclusion The allelic polymorphism of HLA-A, B and DRB1 loci of Han race population in Lanzhou area was between North and South Han race of China, close to Northwest China Hui, and markedly different to Northwest China Uigur and Tibetan race populations.
出处
《中华医学遗传学杂志》
CAS
CSCD
北大核心
2006年第1期89-91,共3页
Chinese Journal of Medical Genetics
