摘要
目的研究体外培养的人牙囊细胞的特征,以及在不同流体静压力作用下细胞程序性死亡(PCD)的改变,探讨PCD在牙齿萌出过程中的作用。方法取12岁男孩埋伏阻生牙齿的牙囊进行人牙囊细胞培养,将传代的培养细胞应用TUNEL的方法标记PCD细胞,分别在50kPa和100kPa流体静压力作用1h后观察PCD变化,以不加压的人牙囊细胞作为对照。结果体外培养的人牙囊细胞呈梭形、纺锤形或多角形,具有成纤维细胞特征。在50kPa流体静压力下人牙囊细胞PCD阳性细胞率与对照组无统计学差异(P>0.05),而100kPa流体静压力下PCD阳性细胞率较对照组增加了31.65%,有统计学差异(P<0.01)。结论流体静压力可以影响体外培养的人牙囊细胞的程序性死亡。
Objective Tooth eruption requires the presence of the dental follicle （DF） around the unerupted tooth. This study is to investigate programmed cell death on human dental follicle cells and changes of programmed ceil death under different hydrostatic pressures： 0, 50 and 100 kPa. Methods Human dental follicles from third mandibular molars were surgically removed from adolescents who need for orthodontics treatment after informed content, then trypsinized and cultured. Human dental follicle cells were divided into three groups according to different hydrostatic pressures： 0, 50 and 100 kPa and their programmed ceil death were labeled by using TdT-medi-ated- dUTP nick and labeling（TUNEL）. Results Dental follicle cells cultured were elongate shape and exhibited fibroblestic characteristics. Compared with 0 kPa, programmed ceil death cells on human dental follicle ceils were increased 0.23% and 31.65% under 50kPa and lOOkPa hydrostatic pressures respectively, lOOkPa group increased signnificantly （P〈0.01）. Conclusion It suggested that programmed ceil death occured in human dental follicle ceils cultured in vitro and was influenced by different hydrostatic pressures. Hydrostatic pressure may improve tooth erup-tion through dental follicle.
出处
《华西口腔医学杂志》
CAS
CSCD
北大核心
2006年第5期466-468,共3页
West China Journal of Stomatology
基金
国家自然科学基金资助项目(30400510)
关键词
人牙囊细胞
细胞程序性死亡
流体静压力
human dental follicle cells
programmed cell death
hydrostatic pressure
