摘要
目的：观察和分析细胞程序性死亡（ＰＣＤ）在细胞凝聚区形成中的作用和意义。方法：建立和制备Ｃ５７ＢＬ／６Ｎ系小鼠胚胎期１３、１４、１５、１６ｄ和生后１０ｄ等多时间点的颌面部发育模型，采用原位末端转移酶标记法标记颌面部组织的ＰＣＤ细胞。结果：在颌面部骨、牙齿等多种组织器官形成初期都包括间充质细胞的聚集，并出现特定的细胞凝聚区。随着细胞凝聚的出现，ＰＣＤ的数量和密度增多。当凝聚区内的细胞向特定细胞系表型转化时，ＰＣＤ数量下降。结论：ＰＣＤ在颌面部凝聚区的形成中发挥着重要的作用。
Objective: To observe and analyse the role and effects of programmed cell death (PCD) in the formation of cellular condensation in maxillofacial development. Method: The model of maxillofacial developent of C57BL/6N mouse was established and the series samples of 13, 14, 15 and 16 days of gestation and that of 10 days after birth were processed. PCD was labelled by using TdT mediated dUTP nick end labeling (TUNEL). Results: It was observed that condensation was involved in the development of bone, tooth and other tissues in maxillofacial region. The amount of PCD-positive cells increased after the cell aggregation initialed, in contrast, the number of PCD positive cell decreased when the cells in condensation were undergoing specialized differentiation. Conclusion: PCD is a critical factor in the formation of cellular condensation and PCD contributes in both controlling of the quality and quantity of cells in the condensation and the remoudling of the condensation.
出处
《实用口腔医学杂志》
CAS
CSCD
北大核心
1999年第1期42-44,共3页
Journal of Practical Stomatology
关键词
颌面部
细胞凝聚区
细胞凋亡
胚胎
Programmed cell death
Stem cells
Jaw
Face
Embryo
