摘要
目的建立显脉香茶菜中冬凌草甲素的HPLC含量测定方法。方法用KromasilC18色谱柱(250mm×4.0mm,5μm);流动相:甲醇-水(45∶55);体积流量:1mL/min;检测波长:235nm;柱温30℃;分析时间为20min。结果冬凌草甲素在茎和叶中含量分别为0.047mg/g和0.791mg/g,茎中含量约相当于叶中含量的1/17。冬凌草甲素平均回收率为98.33%。结论该方法快速、方便、分离度好,其他成分无干扰,适用于显脉香茶菜药材中冬凌草甲素的含量测定。
Objective To develop a HPLC method for the determination of oridonin in the leaves and stems of Rabdosia nervosa. Methods All of reference substances and samples were separated with the mobile phase of methanol： water （45 ： 55） under isocratic elution for 20 min on a Kromasil C18 reversed - phase column （250 mm × 4.0 mm, 5 μm） , the flow rate was 1.0 mL · min^-1, the detection wavelength was 235 nm, and the column temperature was 30 ℃. Results The content of oridonin was 0. 047 mg/g in stems and 0. 791 mg/g in leaves, the content in stems being about one seventeenth of that in leaves. The average recovery of oridonin was 98.33 %. Conclusion This method is simple, sensitive, accurate and suitable for quantitative determination of oridonin in Rabdosia nervosa.
出处
《中药新药与临床药理》
CAS
CSCD
2006年第5期354-356,共3页
Traditional Chinese Drug Research and Clinical Pharmacology
