摘要
以（８６）Ｒｂ示踪法，观察３种肿瘤细胞的钠系转运活性及冬凌草甲素（Ｏｒｉｄ．）对其的影响。结果表明：（Ｏｒｉｄ．）腹腔注射及体外作用均能显著抑制艾氏腹水瘤细胞的钠系转运功能；体外作用１２小时，半数抑制浓度约为５μｇ／ｍｌ，在此浓度下，药物作用１小时的抑制率为２２％。同时测定人癌细胞系７９０１，Ｈｅｐ－２的钠泵活性高于人胚肺纤维母细胞系ＳＬ，且对Ｏｒｉｄ．较敏感。本文结果提示Ｏｒｉｄ．抗癌机理可能与钠泵活性抑制有关，从生物膜转运角度进一步证明Ｏｒｉｄ．的抗肿瘤作用。
The sodium pump activity of tumor cells and effect of ocdonin (Orid. ) were measured by using 86Rb trancing method. The results showed that serum stimulus increased pump activity of EAC cell in vitrol while Orid inhibited the 86Rb infulx of EAC cell in vitro (1-7.5μg/ml) and in vivo (5-20mg/ (kg. d) × 3). preincubating cell with Orid (5ug/ml) for 1, 3, 6, 9b, the inhibitory rates were 22%, 39%, 50%, 63%, respectively. The pump activity of tumor cell lines 7901, Hep2 and normal cell line SL were 4255, 4681 and 2632 ; the inhibitiry rates of Orid. (5ug/ml) were 42%, 51% and 20% separately. These data suggested that Orid. can restrain tumor cell growth by means of inhibiting sodium pump activity.
出处
《肿瘤防治研究》
CAS
CSCD
北大核心
1994年第4期208-209,共2页
Cancer Research on Prevention and Treatment
