摘要
将虎杖(Polygonum cuspidatum)不同外植体经不同消毒时间处理后,接种在添加不同激素种类和水平的相同基本培养基上或相同激素种类和水平基本培养基上进行诱导实验,同时对根、根茎芽、叶、韧皮诱导的愈伤组织进行白藜芦醇的含量测定实验,其结果表明:基本培养基以MS较好,外植体叶对激素种类较为敏感,其中适当浓度的NAA诱导愈伤组织比2,4-D的效果要好,KT比BA要好,在有KT存在的培养基上诱导愈伤组织比较紧密,有利于分化;在MS+NAA2m g/L+KT0.1mg/L培养基上诱导愈伤组织较好,根茎芽的诱导率最高,为73%.愈伤组织的生长趋势从接种的第3d开始生长,到21d时生长达到最高峰,干重为0.461g,以后生长速度减慢.不同材料诱导的愈伤组织中白藜芦醇的含量以根茎部芽的愈伤组织含白藜芦醇最高,其次是叶和根,最低的为韧皮.
Different explants of Polygonum cuspidatum after treatment of different antisepsis time were inoculated on same elementary culture medium added different varieties and levels of phytohormone or different elementary culture medium added same varieties and levels of phytohormone. The results showed that： MS was the best elementary culture medium, leaf explant was more sensitive to phytohormone, in which effects on calli inducement of NAA were better than 2, 4-D and KT better than BA. Calli inducement on culture medium with KT were compactness which was benefit to differentiation. Divided culture medium containing MS and NAA 1.0 mg/L and KT 1.5 mg/L had good effect of re-divided onplant stem. Culture medium containing MS and NAA 2 mg/L and KT 0. 1 mg/L had good inducement effect on calli, its inducing rate on rhizome with bud was the highest reaching up to 73%. The growing trend of callus began to grow after inoculating three days. and the growth of it came to the tiptop in the twenty-one day, its dry weight had the maximum, the following were leaves and roots, and the bast had the minimum in the different callus.
出处
《生命科学研究》
CAS
CSCD
2006年第3期270-275,共6页
Life Science Research
基金
湖南省科技厅资助项目(03JZY3020)
关键词
虎杖
愈伤组织
白藜芦醇
Polygonum cupidatum
callus
resveratrol
