摘要
为了利用细胞工程开发虎杖悬浮细胞培养、积累白藜芦醇,必须探索虎杖松散型愈伤组织的培养基及其培养条件.以灭菌虎杖茎段、叶片、茎尖等为外植体,探讨9种激素组合在琼脂和珍珠岩固体培养基上诱导愈伤组织的出愈时间、出愈率、在继代培养中分化状况和白藜芦醇含量变化.结果表明:珍珠岩作为支撑物,诱导的愈伤组织比琼脂好;含有NAA或KT的培养基,诱导形成的愈伤组织在继代中均可分化;①号培养基(MS+2,4-D 4.0 mg/L+6-BA 2.0 mg/L+3%蔗糖)对幼叶、茎段和茎尖等都有出愈时间早、出愈率高和愈伤组织生长速度快、松散,适宜于悬浮细胞的培养;愈伤组织白藜芦醇的含量与其外植体来源呈正相关,其中,由幼茎形成的愈伤组织白藜芦醇含量(鲜重)最高,达到160μg/g.
In order to make use of suspension cells from Polygonum cuspidatum to accumulate resveratrol,the medium and culture condition of relaxed callus of Polygonum cuspidatum would be optimized. Stem axis,young leaves and stem tips were acted as explants to induce callus of Polygonum cuspidatum in the medium containing MS with 9 kinds of plant hormone combination,which agar and perlite were used as solid components;and then callus development time,callus development rate ,callus differentiation during subculture and callus resveratrol content were studied. The results are shown that,the callus induced in medium with perlite is better than with agar;the callus can be differentiated into tissues from the medium containing NAA or KT during subculture;in medium （1） with MS＋2,4-D 4.0 mg/L＋6-BA 2.0 mg/L,callus development time is short,and callus development rate is high,and callus is relaxed,and callus development speed is quick from explants of stem axis ,young leaves and stem tips ,while it can be fit for suspension culture ;there is positive relationship between callus resveratrol contents and their origin of their explants,thereinto,the resveratrol content of callus from stem axis induction is 160 μg/g wet weight,and it is the highest content.
出处
《天津科技大学学报》
CAS
2009年第2期9-12,共4页
Journal of Tianjin University of Science & Technology
基金
天津市应用基础及前沿技术研究计划项目(08JCZDTC15300)
关键词
虎杖
白藜芦醇
愈伤组织
植物激素
珍珠岩
Polygonum cuspidatum
resveratrol
callus
plant hormone
perlite
