摘要
目的为扩大和提高锁阳资源的利用效率。方法以锁阳肉质茎的不同组织部位作为外植体,以MS为基本培养基,筛选诱导愈伤组织产生的最佳外植体。结果以MS为基本培养基附加2,4-D 2.0 mg/L、6-BA 1.0 mg/L和NAA1.0 mg/L,在暗培养、20℃条件下锁阳肉质茎的维管组织部分是诱导愈伤组织的最佳外植体,髓部较差,鳞片叶未诱导出愈伤组织。结论初步筛选出了适宜锁阳愈伤组织诱导的外植体和培养条件。
Objective To explore and improve the utilization efficiency of Cynomorium songaricum resources. Methods The different tissue parts of fleshy stem of C.songaricum were determined as the explants and cultured on MS culture media in different culture condition for callus induction. Results The vascular tissue parts of fleshy stem of C.songaricum was optimum explants of callus induction,the following was pith tissue,while scale leaf was relatively bad when MS as basic media and adding 2,4-D（2.0 mg/L）,6-BA（1.0 mg/L） and NAA（1.0 mg/L） by growing in the dark at 20℃. Conclusion The optimized explants and culture condition of callus induction of C.songaricum is obtained.
出处
《时珍国医国药》
CAS
CSCD
北大核心
2012年第1期66-67,共2页
Lishizhen Medicine and Materia Medica Research
基金
宁夏回族自治区自然科学基金(No.NZ10109)
宁夏医科大学面上项目(No.XM2011006)
关键词
锁阳
肉质茎
愈伤组织
最佳外植体
Cynomorium songaricum Rupr Fleshy stem Callus Optimal explant
