摘要
作者旨在对骨形态生成蛋白／脱钙骨基质（ＢＭＰ／ＤＢＭ）复合体在关节软骨缺损修复中的作用进行探讨。在１６只雄性兔双侧股骨髁间凹各做一直径４ｍｍ，深２．５ｍｍ的全层关节软骨缺损。１６个右膝缺损区植入ＢＭＰ／ＤＢＭ，８个左膝缺损内单独植入ＤＢＭ，另外８个左膝为空白。术后１２周处死动物，取材，石蜡包埋，连续切片，做苏木素一伊红，甲苯胺蓝及ｓａｆｒａｎｉｎ－Ｏ染色。结果显示，在ＢＭＰ／ＤＢＭ组，植入物完全被吸收并被新生软骨替代，１４例（１４／１６）愈合，且１２例ｓａｆｒａｎｉｎ－Ｏ阳染，新生软骨厚度和特征与邻近正常软骨相似。ＤＢＭ组只有２例（２／８）愈合，ｓａｆｒａｎｉｎ－Ｏ染色全部阴性。空白组未见新生软骨。结果说明，ＢＭＰ／ＤＢＭ能在关节腔内诱导软骨再生，并能以透明软骨修复全层关节软骨缺损。
Theauthorsstudiedtheefectsofbonemorphogeneticprotein/demineralizedbonema-trix(BMP/DBM)fortherepairofdefectsofthearticularcartilage.In16malerabbits,defectsof4mmdiameterand2.5mmdepthwerecreatedthroughfulthicknessofthearticularcartilagebilateralyintheintercondylargrooveofthefemur.BMP/DBMwasinsertedin16oftherightkneedefects.Overtheleftside,DBMdiscsweregraftedalone,andinanother8,thedefectswereleftaloneascontrols.At12weekspostoperatively,theanimalsweresacrificedandspecimensofthedistalfemurwereprocuredandpreparedforhistologicstudywithHEstain,toluidineblueandsafranin-Ostaining.IntheBMP/DBMgraftedgrouptheresultsshowedcompleteabsorptionofBMP/DBMandsubstitutionbynewcartilagetissue.14ofthe16kneedefectswerehealedand12hadpositivestainingwithsafranin-O.Thethick-nessandfeaturesofthenewarticularcartilagewassimilartothatofthenormal.InDBMgraftedgroup,thethicknessofthenewarticularcartilagewasnotashealthyasintheBMP/DBMgraftedgroup.Noneofthespecimenwaspositvelystainedwithsafranin-O.Only2ofthe8defectshealed.Inthecontrols,nonewcartilagetissuewasseen.Conclusively,BMP/DBMmayinducecartilageregenera-tionandrepairthearticularcartilagedefectswithhyalinecartilageoverthejointsurface.
出处
《中华骨科杂志》
CAS
CSCD
北大核心
1996年第9期578-580,共3页
Chinese Journal of Orthopaedics
