摘要
目的:研究骨髓间质干细胞向肌样细胞分化前后骨骼肌特异性基因的表达。方法:体外分离成年SD大鼠骨髓干细胞,培养增殖,用5-氮杂胞苷诱导分化,RT-PCR检测分化前和分化后1d、2d、5d和7d骨骼肌特异性转录因子生肌决定因子MyoD、肌细胞生成素myogenin、MRF4以及肌肉特异性肌酸磷酸激酶MCK的表达。结果:MyoD在诱导前后均有表达,在诱导后1d表达显著上调(P<0·05);myogenin、MRF4和MCK在诱导前无表达,诱导后2d开始出现myogenin和MRF4的表达,诱导后7d开始出现MCK的表达。结论:骨髓间质干细胞能表达一定水平的骨骼肌细胞的分化调控基因MyoD,它向肌样细胞的分化可能与MyoD、myogenin和MRF4有关。
AIM： To analyze skeletal muscle specific gene expression in rat bone marrow mesenchymal stem cells （MSCs） differentiating into muscle- like cells. METHODS： The MSCs from adult SD rat were isolated, cultured and passaged in vitro by routine culture technique. Then, they were induced and differentiated with 5 - azacytidine. The expression of skeletal muscle specific transcription factors, MyoD, myogenin, MRF4 and muscle specific creatine kinase （MCK） were detected before and 1, 2, 5, 7 d after induction by reverse transcription polymerase chain reaction （ RT - PCR ）. RESULTS ： The expression of MyoD was detected before and after induction, its expression was markedly up - regulated 1 d after induction （ P 〈 0. 05 ）. The expression of myogenin, MRF4 and MCK was not detected before induction. The expression of myogenin and MRF4 was detected 2 d after induction initially. The expression of MCK was detected 7 d after induction initially. CONCLUSION： MSCs can express skeletal muscle specific differentiating control gene MyoD to some extent. The differentiation of MSCs into muscle - like ceils may be related to MyoD, myogenin and MRF4.
出处
《中国病理生理杂志》
CAS
CSCD
北大核心
2006年第9期1665-1668,共4页
Chinese Journal of Pathophysiology
基金
国家自然科学基金资助项目(No.30370510)
广东省自然科学基金资助项目(No.31693)
卫生部临床重点项目(No.2001321)
关键词
骨髓间质干细胞
细胞分化
基因表达
阿扎胞苷
肌样细胞
Bone marrow mesenchymal stem cells
Cell differentiation
Gene expression
Azacytidine
Muscle - like cells
