摘要
目的 :体外培养并诱导大鼠骨髓间质干细胞 (MSCs)分化为肌样细胞。方法 :采用常规技术对SD鼠MSCs进行体外培养传代、鉴定、诱导分化和免疫组化、透射电镜检测分析。结果 :流式细胞仪检测 ,细胞表达CD2 9和CD4 4 ,不表达CD11b和CD4 5 ;经一定浓度 5 -氮杂胞苷和两性霉素B诱导分化后细胞desmin和myoglobin染色阳性 ;电镜观察肌样细胞胞浆靠胞膜缘可见无细胞器的条状肌丝区带。结论 :传代贴壁生长的梭形细胞为MSCs。MSCs可能具有表达肌细胞的特异性启动或分化调控基因 ;5 -氮胞苷等化合物可使DNA的胞嘧啶去甲基化 ,从而诱导MSCs向肌源性细胞分化。临床有运用MSCs治疗肌萎缩性疾病的前景。
AIM:To study the differentiation of rat marrow-derived mesenchymal stem cells(MSCs) into myoid cells in vitro . METHODS:The MSCs of SD rat were cultured?passaged?induced and differentiated in vitro used by routine culture technique, and evaluated by FACScan flow cytometer,detected by immunohistochemistry and analyzed by Hitachi H-600 transmission electron microscope(TEM). RESULTS:FACScan shows that cells expressed the antigens of CD29 and CD44, not those of CD11b and CD45; cells show positive response of staining with desmin and myoglobin after processing by two compounds of 5-azacytidine and amphotericin B. There were stripform zone of myofilament without any organells beside the edge of membrane in myoid cell of induction and differentiation checked by TEM. CONCLUSION: The passaged cells were MSCs and the MSCs may have specific gene structures that can differentiate into myoid cells. The demethylation or hypomethylation may conduct by compounds of 5-azacytidine and amphotericin B, which could be involved in activating phenotype-specific genes to differentiate MSCs into myoid cells. There are good outlook on clinical treatment of illness of myatrophy using by MSCs.
出处
《中国病理生理杂志》
CAS
CSCD
北大核心
2003年第9期1165-1169,共5页
Chinese Journal of Pathophysiology
基金
国家自然科学基金资助项目 (No .30 2 70 732
30 0 0 0057
30170 337)
广州市科学技术局基金资助项目(2 0 0 2J1-C0182 )
关键词
骨髓
干细胞
肌
大鼠
Bone marrow
Stem cells
Muscles
Rats
