摘要
用体外培养的骨髓间充质干细胞(bone marrow mesenchymal stem cells,BMMSCs)治疗深Ⅱ度烫伤大鼠。体外培养雄性Wistar大鼠BMMSCs,流式细胞仪鉴定。将BMMSCs分别通过创面局部、尾静脉注射移植给深Ⅱ度烫伤的雌性大鼠。观察创面愈合情况;不同时间点取组织标本制作石蜡切片,HE染色观察;PCR检测创面皮肤组织和骨髓中供体鼠Y染色体基因性别决定区(Sry)片段的表达。结果显示,静脉注射组和创面局部注射组愈合速度均快于对照组(P<0.05),但两治疗组之间比较差异无显著性(P>0.05)。HE染色结果显示治疗组创面愈合质量均好于对照组。两治疗组动物创面组织及静脉注射组骨髓组织均有Y染色体基因的表达。因此,大鼠供体骨髓MSCs对其受体皮肤深Ⅱ度烫伤具有一定的治疗作用。烫伤促使BMMSCs向创面迁移并参与烫伤创面愈合。
Bone marrow mesenchymal stem cells （BMMSCs） cultured in vitro were used to treat the rats of deep partial thickness derma burn injury, Bone marrow-derived MSCs of male Wistar rats were isolated and cultured in vitro routinely and the 3rd passage was taken for identification of specific surface antigens by flowcytometry. The BMMSCs were injected into the female rats of deep partial thickness derma burn injury model through the wound sites （group A） or vena caudalis （group B）. Then the rate of wound healing was observed. At the 7th, 14th, 21 st, 28th day after burn injury, wound skin tissues were harvested and routinely paraffin-embedded and sectioned. The sections were stained with hematoxylin and eosin （HE） for histological examination. Furthermore genome DNA extracted from wound skin tissues and the bone marrow harvested was used to detect sry gene of Y chromosome by polymerase chain reaction （PCR）. The results showed that the rate and quality of wound healing of the two treatment groups （group A and B） were better than that of the control group （Group C）, but there was not significantly different between group A and group B. Y chromosome gene （Sry） could be detected in wound skin tissues of female rats transplanted with BMMSCs of male rats and the bone marrow of rats in group B by PCR. These resultssuggested that BMMSCs transplantation is effective in deep partial thickness derma burn injury in rats. It may be possible that BMMSCs can migrate to wounds and play a role during the course of wound healing.
出处
《中国细胞生物学学报》
CAS
CSCD
2010年第1期69-72,共4页
Chinese Journal of Cell Biology
基金
江西省教育厅青年基金资助项目(No.GJJ09432)
南昌大学科技基金(医科类)资助项目~~
关键词
骨髓间充质干细胞
烫伤
创面愈合
多聚酶链式反应
bone marrow mesenchymal stem cells
bum
wound healing
polymerase chain reaction
