摘要
AIM： To investigate the value and prospect of RT-PCR in detecting micrometastasis in regional lymph nodes of gastric cancer. METHODS： Histopathology was used and K19 mRNA expression was detected by RT-PCR in tumor tissues and lymph nodes from gastric cancer patients undergoing radical resection of gastric carcinoma. RESULTS： K19 mRNA was expressed in all tumor specimens of 30 cases; of the 126 lymph nodes, 26 were histopathologically positive （20.6%）, and 42 positive （33.3%） by RT-PCR. Amplification fragments of 460 and 540 bp were shown in all the tumor tissues and metastatic lymph nodes after K19 and β-actin RT-PCR, while only a 540 bp fragment appeared in the lymph nodes of non-tumor patients. CONCLUSION： K19 mRNA RT-PCR is sensitive and specific in testing micrometastasis in regional lymph nodes of gastric cancer, and it is superior to routine histopathology.
瞄准：在在胃的癌症的地区性的淋巴节点检测微转移调查 RT-PCR 的价值和前景。方法：组织病理学说被使用， K19 mRNA 表示被 RT-PCR 从经历胃的癌的激进的切除术的胃的癌症病人在肿瘤纸巾和淋巴节点检测。结果：K19 mRNA 在 30 个案例的所有肿瘤标本被表示；126 个淋巴节点， 26 是组织病理学说的联盟者积极(20.6%) ，并且 42 积极(33.3%) 由 RT-PCR。460 和 540 bp 的扩大碎片在 K19 和贝它肌动朊 RT-PCR 以后在所有肿瘤纸巾和变形淋巴节点被显示出，当 540 仅仅 bp 碎片出现在非肿瘤病人的淋巴节点时。结论：K19 mRNA RT-PCR 在在胃的癌症的地区性的淋巴节点的严峻的微转移敏感、特定，并且它比平淡的组织病理学说优异。
基金
Supported by funds from the Science and Technology Department of Jilin Province, China
