摘要
对2个耐热性不同的水稻品系进行高温处理（8：00～17：00，37℃；17：00～8：00，30℃），测定了高温胁迫对水稻剑叶中保护酶类活性和膜透性的影响。结果表明，水稻剑叶中超氧化物歧化酶（SOD）、过氧化氢酶（CAT）、过氧化物酶（POX）和抗坏血酸过氧化物酶（AsA—POX）活性在高温胁迫初期均有明显增加，尔后快速下降，耐热品系996这4种酶活性的增幅大于热敏感品系4628；热敏感品系4628剑叶中硫代巴比妥酸反应产物（TBARS）含量和膜透性在高温胁迫下增幅大于耐热品系996；剑叶中超氧阴离子（O2^-）产生速率和过氧化氢（H2O5）含量随着高温胁迫时间延长而增加，热敏感品系4628大于耐热品系996。
High temperature is a key factor during rice heading and flowering period affecting seed set rate and grain yield in main production areas of China, but the physiological mechanism was related to high temperature stress remains unclear. The experiments were carried out in growth chambers to investigate the effects of high temperature stress during heading and flowering period on protective enzyme activities and membrane permeability of flag leaf using two rice lines including hightemperature tolerant line 996 and high-temperature sensitive line 4628. Seven day/night temperature （8 ： 00-17 ： 00, 37 ℃ ; 17：00- 8 ： 00, 30℃ ） after heading were established. The results showed that superoxide dismutase （ SOD）, catalase （CAT）, peroxidase （POX） and ascorbic acid-peroxidase （AsA-POX） activities in flag leaf of rice increased obviously at early stage of high temperature stress, then decreased rapidly. The increase of the enzyme activities in high-temperature tolerant line 996 was greater than that in high-temperature sensitive 4628. The increase of thiobarbituric acid reaction substance（TBARS） content and the membrane permeability in the line 4628 was greater than that in the line 996. The production rate of superoxide anion and the content of hydrogen peroxide （H2 O2 ） increased during the stress period, which was greater in the line 4628 than in the line 996. The above results indicated that protective enzyme activities declined and membrane permeability increased under high temperature stress, and high-temperature sensitive line 4628 was affected more by high temperature stress than high-temperature tolerant line 996.
出处
《作物学报》
CAS
CSCD
北大核心
2006年第9期1306-1310,共5页
Acta Agronomica Sinica
基金
国家高技术研究发展计划(863计划)现代农业技术项目(2001AA241023)
国家自然科学基金资助项目(3015004)
关键词
水稻
高温胁迫
剑叶
保护酶
膜透性
Rice
High temperature stress
Flag leaf
Protective enzyme
Membrane permeability
