摘要
为确立水杨酸对银杏Ginkgo biloba的耐热性调控效果并探讨外源水杨酸下银杏耐热的可能机制,分别对2年生银杏叶片喷施100,200μmol·L-1的水杨酸溶液处理48h,并以喷施蒸馏水为对照,然后以44℃的高温胁迫4h。结果表明:高温胁迫下,2种浓度的水杨酸溶液均可抑制银杏叶片中相对电导率和丙二醛质量摩尔浓度的升高,促进超氧化物歧化酶活性的增强和抗坏血酸质量分数的升高,而且与对照相比,外源水杨酸处理过的银杏叶片可溶性蛋白质量分数较高,可溶性糖较低。各项生理生化指标均反映了水杨酸对缓解高温对银杏的伤害起到了一定作用;100μmol·L-1的水杨酸溶液比200μmol·L-1的水杨酸溶液缓解银杏受高温伤害的效果要好。
To determine tolerance, Ginkgo biloba seedlings were sprayed with salicylic acid （SA） and then subjected to high-temperature stress. Electrical conductivity, malondialdehyde （-MDA） content, superoxide dismutase（SOD） activity, ascorbic acid（ASA） and soluble proteins and carbohydrates were determined. The 2 year-old seedlings were sprayed by SA at 0（the control）, 100 or 200 μmol·L^-1 for 4 times （twice for one day） in July of 2007. Results showed that： SA at 100 μmol·L^-1 increased SOD activity and ASA content, significantly （P〈0.05）more efficient than the control and 200 μmol·L^-1; SA at 100 and 200 μmol·L^-1 restrained（P〈0.05） the increase of electrical conductivity, significantly（P〈0.05） lower than the control. In addition, compared with the control, treated seedling leaves showed higher （P〈0.05） soluble protein content and lower （P〈0.05） soluble carbohydrates content. All of these results indicated that pretreatment with SA could increase the tolerance of G. biloba seedlings to high-temperature stress. [Ch, 3 fig. 21 ref.]
出处
《浙江林学院学报》
CSCD
北大核心
2008年第6期756-759,共4页
Journal of Zhejiang Forestry College
基金
科技部林业科技支撑项目(2006BAD18B03)
关键词
植物学
银杏
水杨酸
高温胁迫
生理生化效应
botany
Ginkgo biloba
salicylic acid （SA）
high-temperature stress
physio-biochemical effect
